Ermakov Evgeny A, Smirnova Ludmila P, Parkhomenko Taisiya A, Dmitrenok Pavel S, Krotenko Nina M, Fattakhov Nikolai S, Bokhan Nikolay A, Semke Arkadiy V, Ivanova Svetlana A, Buneva Valentina N, Nevinsky Georgy A
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia Novosibirsk State University, 2 Pirogova Street, Novosibirsk 630090, Russia.
Mental Health Research Institute, Russian Academy of Medical Sciences, 4 Aleutskaya Avenue, Tomsk 634014, Russia.
Open Biol. 2015 Sep;5(9):150064. doi: 10.1098/rsob.150064.
It is believed that damage to the membranes of brain cells of schizophrenia (SCZ) patients induces the formation of autoantigens and autoantibodies. Nevertheless, the importance of immunological changes leading to the loss of tolerance to self-antigens in the genesis of SCZ has not been established. The MALDI mass spectra of the IgG light chains of 20 healthy donors were relatively homogeneous and characterized by one peak with only one maximum. In contrast to the healthy donors, the MALDI mass spectra of IgG light chains corresponding to 20 SCZ patients demonstrated, similarly to 20 autoimmune systemic lupus erythematosus (SLE) patients, two maxima of a comparable intensity. In addition, the MALDI spectra of the IgG light chains of five SLE and four SCZ patients contained a small additional brightly pronounced peak with remarkably lower molecular mass compared with the main one. DNase autoantibodies (abzymes) can be found in the blood of patients with several autoimmune diseases, while the blood of healthy donors or patients with diseases without a significant disturbance of the immune status does not contain DNase abzymes. Here, we present the first analysis of anti-DNA antibodies and DNase abzymes in the sera of SCZ patients. Several strict criteria have been applied to show that the DNase activity is an intrinsic property of IgGs from the sera of SCZ patients. The sera of approximately 30% of SCZ patients displayed a higher content of antibodies (compared with 37% of SLE) interacting with single- and double-stranded DNA compared with healthy donors. Antibodies with DNase activity were revealed in 80% of the patients. These data indicate that some SCZ patients may show signs of typical autoimmune processes to a certain extent.
人们认为，精神分裂症（SCZ）患者脑细胞的膜损伤会诱导自身抗原和自身抗体的形成。然而，在SCZ发病过程中导致对自身抗原耐受性丧失的免疫变化的重要性尚未确定。20名健康供体的IgG轻链的基质辅助激光解吸电离质谱（MALDI）相对均匀，其特征是只有一个最大值的一个峰。与健康供体相比，20名SCZ患者对应的IgG轻链的MALDI质谱显示，与20名自身免疫性系统性红斑狼疮（SLE）患者类似，有两个强度相当的最大值。此外，5名SLE患者和4名SCZ患者的IgG轻链的MALDI光谱包含一个额外的小峰，与主峰相比，其分子量明显更低且明显突出。在几种自身免疫性疾病患者的血液中可以发现脱氧核糖核酸酶自身抗体（催化抗体），而健康供体或免疫状态无明显紊乱的疾病患者的血液中不含有脱氧核糖核酸酶催化抗体。在此，我们首次分析了SCZ患者血清中的抗DNA抗体和脱氧核糖核酸酶催化抗体。已经应用了几个严格的标准来表明脱氧核糖核酸酶活性是SCZ患者血清中IgG的固有特性。与健康供体相比，约30%的SCZ患者血清中与单链和双链DNA相互作用的抗体含量更高（与37%的SLE患者相比）。在80%的患者中发现了具有脱氧核糖核酸酶活性的抗体。这些数据表明一些SCZ患者可能在一定程度上表现出典型自身免疫过程的迹象。
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