猫后睾吸虫感染的肝脏中的疟色素“结节”
Hemozoin "knobs" in Opisthorchis felineus infected liver.
作者信息
Pershina Alexandra G, Saltykova Irina V, Ivanov Vladimir V, Perina Ekaterina A, Demin Alexander M, Shevelev Oleg B, Buzueva Irina I, Gutakovskii Anton K, Vtorushin Sergey V, Ganebnykh Ilya N, Krasnov Victor P, Sazonov Alexey E, Ogorodova Ludmila M
机构信息
Siberian State Medical University, 2, Moskovsky trakt, 634050, Tomsk, Russia.
National Research Tomsk Polytechnic University, 30, Lenina Ave, Tomsk, 634050, Russia.
出版信息
Parasit Vectors. 2015 Sep 17;8:459. doi: 10.1186/s13071-015-1061-5.
BACKGROUND
Hemozoin is the pigment produced by some blood-feeding parasites. It demonstrates high diagnostic and therapeutic potential. In this work the formation of co-called hemozoin "knobs" - the bile duct ectasia filled up by hemozoin pigment - in Opisthorhis felineus infected hamster liver has been observed.
METHODS
The O. felineus infected liver was examined by histological analysis and magnetic resonance imaging (MRI). The pigment hemozoin was identified by Fourier transform infrared spectroscopy and high resolution electrospray ionization mass spectrometry analysis. Hemozoin crystals were characterised by high resolution transmission electron microscopy.
RESULTS
Hemozoin crystals produced by O. felineus have average length 403 nm and the length-to-width ratio equals 2.0. The regurgitation of hemozoin from parasitic fluke during infection leads to formation of bile duct ectasia. The active release of hemozoin from O. felineus during in vitro incubation has also been evidenced. It has been shown that the hemozoin knobs can be detected by magnetic resonance imaging.
CONCLUSIONS
In the paper for the first time the characterisation of hemozoin pigment extracted from liver fluke O. felineus has been conducted. The role of hemozoin in the modification of immune response by opisthorchiasis is assumed.
背景
疟色素是一些吸血寄生虫产生的色素。它具有很高的诊断和治疗潜力。在这项研究中，观察到在感染猫后睾吸虫的仓鼠肝脏中形成了所谓的疟色素“结节”——由疟色素填充的胆管扩张。
方法
通过组织学分析和磁共振成像（MRI）检查感染猫后睾吸虫的肝脏。通过傅里叶变换红外光谱和高分辨率电喷雾电离质谱分析鉴定疟色素。用高分辨率透射电子显微镜对疟色素晶体进行表征。
结果
猫后睾吸虫产生的疟色素晶体平均长度为403纳米，长宽比为2.0。感染期间寄生吸虫的疟色素反流导致胆管扩张。体外培养期间也证实了猫后睾吸虫疟色素的活性释放。研究表明，磁共振成像可以检测到疟色素结节。
结论
本文首次对从肝吸虫猫后睾吸虫中提取的疟色素进行了表征。推测疟色素在华支睾吸虫病免疫反应调节中的作用。
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