Arias de Luxán J, Martínez Ramírez A, Sánchez Salgado G, López Sánchez A, Hernández Vallejo G
Av Odontoestomatol. 1989 Oct;1(2):102-8.
Sixteen teeth from patients with advanced periodontal disease were processed with the direct fixative technique for observation with a Scanning Electron Microscope in order to test the adequacy of this technique and to observe the components of the tooth wall of the periodontal pocket. Different cell populations are described emphasizing the appearance of intact neutrophils. The results suggest that this fixative method may be an improvement on the conventional techniques for SEM.
为了测试直接固定技术的适用性并观察牙周袋牙壁的组成成分，对16颗晚期牙周病患者的牙齿采用直接固定技术进行处理，以便用扫描电子显微镜进行观察。文中描述了不同的细胞群体，重点强调了完整中性粒细胞的外观。结果表明，这种固定方法可能是对扫描电子显微镜常规技术的一种改进。
