Kato Daichi, Suzuki Yutaka, Haga Satoshi, So KyoungHa, Yamauchi Eri, Nakano Miwa, Ishizaki Hiroshi, Choi Kichoon, Katoh Kazuo, Roh Sang-Gun
Lab of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Sendai.
Grassland Management Research Division, NARO Institute of Livestock and Grassland Science, Nasushiobara, Japan.
Anim Sci J. 2016 Apr;87(4):584-90. doi: 10.1111/asj.12448. Epub 2015 Sep 21.
This study aimed to identify the genes associated with the development of the rumen epithelium by screening for candidate genes by digital differential display (DDD) in silico. Using DDD in NCBI's UniGene database, expressed sequence tag (EST)-based gene expression profiles were analyzed in rumen, reticulum, omasum, abomasum and other tissues in cattle. One hundred and ten candidate genes with high expression in the rumen were derived from a library of all tissues. The expression levels of 11 genes in all candidate genes were analyzed in the rumen, reticulum, omasum and abomasum of nine Japanese Black male calves (5-week-old pre-weaning: n = 3; 15-week-old weaned calves: n = 6). Among the 11 genes, only 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), aldo-keto reductase family 1, member C1-like (AKR1C1), and fatty acid binding protein 3 (FABP3) showed significant changes in the levels of gene expression in the rumen between the pre- and post-weaning of calves. These results indicate that DDD analysis in silico can be useful for screening candidate genes related to rumen development, and that the changes in expression levels of three genes in the rumen may have been caused by weaning, aging or both.
本研究旨在通过在计算机上进行数字差异显示（DDD）筛选候选基因，以鉴定与瘤胃上皮发育相关的基因。利用美国国立生物技术信息中心（NCBI）的基因索引数据库中的DDD，分析了牛瘤胃、网胃、瓣胃、皱胃及其他组织中基于表达序列标签（EST）的基因表达谱。从所有组织的文库中获得了110个在瘤胃中高表达的候选基因。分析了9头日本黑毛雄性犊牛（5周龄断奶前：n = 3；15周龄断奶犊牛：n = 6）的瘤胃、网胃、瓣胃和皱胃中所有候选基因中11个基因的表达水平。在这11个基因中，只有3-羟基-3-甲基戊二酰辅酶A合酶2（HMGCS2）、醛酮还原酶家族1成员C1样蛋白（AKR1C1）和脂肪酸结合蛋白3（FABP3）在犊牛断奶前后瘤胃中的基因表达水平有显著变化。这些结果表明，计算机上的DDD分析可用于筛选与瘤胃发育相关的候选基因，并且瘤胃中三个基因表达水平的变化可能是由断奶、年龄增长或两者共同引起的。
