小鼠Lewis肺癌生长和转移过程中活性基质金属蛋白酶与酶原的比例

Ratio of Active Matrix Metalloproteinases and Proenzymes during Growth and Metastasizing of Mouse Lewis Lung Adenocarcinoma.

作者信息

Kisarova Ya A, Kaledin V I, Bogdanova L A, Korolenko T A

机构信息

Research Institute of Physiology and Fundamental Medicine, Novosibirsk, Russia.

Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2015 Aug;159(4):486-9. doi: 10.1007/s10517-015-2999-1. Epub 2015 Sep 22.

DOI:10.1007/s10517-015-2999-1

PMID:26392281

Abstract

Ratio between proMMP and active MMP was studied in the dynamics of growth of the Lewis lung adenocarcinoma with lung metastasis. It was shown that tumor growth is associated with an increase in the content of proMMP (day 20; terminal stage), but the level of active MMP in tumor tissue did not signifi cantly change. The development of lung metastasis was accompanied by accumulation of active MMP (days 7, 15, and 20) and a decrease in the content of pro-MMP (days 7, and 20) in comparison with the control. In the spleen of these mice (metastasis-free organ), an increase in the levels of proMMP (day 20) and especially active MMP (days 7, 15, and 20) were found. The results suggest that tumor development shifts the proportion between active MMP and proenzymes in the tumor, lungs with metastasis, and spleen without metastasis.

摘要

在伴有肺转移的Lewis肺癌生长动态过程中，对前基质金属蛋白酶（proMMP）与活性基质金属蛋白酶（MMP）之间的比例进行了研究。结果显示，肿瘤生长与proMMP含量增加有关（第20天；终末期），但肿瘤组织中活性MMP水平无显著变化。与对照组相比，肺转移的发展伴随着活性MMP的积累（第7天、15天和20天）以及pro-MMP含量的降低（第7天和20天）。在这些小鼠的脾脏（无转移器官）中，发现proMMP水平升高（第20天），尤其是活性MMP水平升高（第7天、15天和20天）。结果表明，肿瘤发展改变了肿瘤、有转移的肺以及无转移的脾脏中活性MMP与酶原之间的比例。