Gopinath M, Mohanapriya C, Sivakumar K, Baskar G, Muthukumaran C, Dhanasekar R
Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Chennai 603308, India.
Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Chennai 603308, India.
Ecotoxicol Environ Saf. 2016 Dec;134(Pt 2):370-376. doi: 10.1016/j.ecoenv.2015.09.015. Epub 2015 Sep 26.
Microbial abatement of toluene using Aspergillus niger in coir packed upflow bioreactor was investigated in this study. Toluene degrading microbes were isolated from municipal sewage effluent and identified by 16s rRNA sequencing method. The microbes were cultured in 2% (v/v) toluene input per day, which exhibited 95% removal efficiency with the kinetic correction value (R) of 0.9024 at the optimum flow rate of about 0.4mh. Various parameters such as effect of flow rate, column height, elimination capacity and EBRT with removal efficiency for 50 day cycle were also optimized. The plug flow model for toluene degradation was properly expressed and the Monod kinetics constant K and r values were determined as 2.25gm and 67.773gmh respectively for microbial growth rate.
本研究考察了在椰壳填充上流式生物反应器中使用黑曲霉对甲苯进行微生物降解的情况。从城市污水中分离出甲苯降解微生物，并通过16s rRNA测序方法进行鉴定。微生物在每天输入2%（v/v）甲苯的条件下培养，在约0.4mh的最佳流速下，去除效率达到95%，动力学校正值（R）为0.9024。还优化了各种参数，如流速、柱高、去除能力和空床停留时间对50天周期去除效率的影响。正确表达了甲苯降解的活塞流模型，微生物生长速率的莫诺德动力学常数K和r值分别确定为2.25gm和67.773gmh。
