Hull S R, Bright A, Carraway K L, Abe M, Hayes D F, Kufe D W
Department of Cell Biology and Anatomy, University of Miami School of Medicine, FL 33101.
Cancer Commun. 1989;1(4):261-7.
The murine monoclonal antibody DF3, prepared against a membrane-enriched fraction of a human breast carcinoma, recognized high molecular weight mucin-like glycoproteins from normal human milk and breast carcinoma cell lines. Although the epitope recognized appeared to be a peptide segment, recognition was altered by neuraminidase, suggesting carbohydrate contributions to antigen recognition. Examination of DF3 antigen isolated from normal human milk and the BT-20 human breast carcinoma cell line showed significant oligosaccharide differences. DF3 antigen from BT-20 cells contained three major oligosaccharides, the peanut agglutinin-binding disaccharide Gal beta 1,3GalNAc, which is the carbohydrate component of the Thomsen-Friedenreich antigen, and its mono-(NeuAc2,3Gal beta 1,3GalNAc and/or Gal beta 1,3(NeuAc alpha 2,6)GalNAc) and disialylated (NeuAc2,3Gal beta 1,3(NeuAc alpha 2,6)GalNAc) derivatives. In contrast, the DF3 antigen from normal human milk contained the tetrasaccharide Gal beta 1,4GlcNASc beta 1,6(Gal beta 1,3)GalNAc as its major neutral oligosaccharide and also sialylated derivatives of the tetrasaccharide, including a monosialo derivative (Gal beta 1,4GlcNac beta 1,6(NeuAc alpha 2,3Gal beta 2,3)GalNAc and/or NeuAc alpha 2,3Gal beta 1,4GlcNAc beta 1,6 (Gal beta 1,3)GalNAc). These results suggest the possibility of carcinoma-associated alterations in O-linked oligosacchardes of cell surface sialomucins and in the activity of the beta 1,6-glucosaminyl transferase involved in mucin biosynthesis.
针对人乳腺癌富含膜成分的部分制备的鼠单克隆抗体DF3，可识别来自正常人乳汁和乳腺癌细胞系的高分子量黏蛋白样糖蛋白。尽管所识别的表位似乎是一个肽段，但神经氨酸酶可改变识别作用，提示碳水化合物对抗原识别有贡献。对从正常人乳汁和BT - 20人乳腺癌细胞系分离的DF3抗原的检测显示，二者存在显著的寡糖差异。来自BT - 20细胞的DF3抗原含有三种主要寡糖，即花生凝集素结合二糖Galβ1,3GalNAc（它是桑福德 - 弗里德赖希抗原的碳水化合物成分）及其单唾液酸化衍生物（NeuAc2,3Galβ1,3GalNAc和/或Galβ1,3(NeuAcα2,6)GalNAc）和双唾液酸化衍生物（NeuAc2,3Galβ1,3(NeuAcα2,6)GalNAc）。相比之下，来自正常人乳汁的DF3抗原含有四糖Galβ1,4GlcNAScβ1,6(Galβ1,3)GalNAc作为其主要中性寡糖，以及该四糖的唾液酸化衍生物，包括一种单唾液酸化衍生物（Galβ1,4GlcNacβ1,6(NeuAcα2,3Galβ2,3)GalNAc和/或NeuAcα2,3Galβ1,4GlcNAcβ1,6 (Galβ1,3)GalNAc）。这些结果提示，细胞表面唾液酸黏蛋白的O - 连接寡糖以及参与黏蛋白生物合成的β1,6 - 氨基葡糖基转移酶的活性可能发生了与癌相关的改变。
