Łukaszewicz Ewa Teresa, Kowalczyk Artur Mikołaj, Rzońca Zenon
Division of Poultry Breeding, Institute of Animal Breeding, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Forestry Wisła; Czarne 6; 43-460 Wisła, Poland.
PLoS One. 2015 Sep 23;10(9):e0138415. doi: 10.1371/journal.pone.0138415. eCollection 2015.
Artificial insemination (AI) is very helpful in solving the reproductive and biodiversity problems observed in small, closed avian populations. The successful production of fertilized eggs using AI is dependent on the collection of good quality semen. Two methods of male sexual stimulation and semen collection from captive kept capercaillie (Tetrao urogallus L.), one of the most seriously endangered grouse species in Europe, are compared in this study. Ejaculates were obtained either with the use of a dummy female or by the dorso-abdominal massage method. Differences in the individual responses of the males to the two methods of semen collection as well as in their semen quality were noted. Only sperm concentration (432.4 x 10(6) mL(-1) with dummy female and 614.5 x 10(6) mL(-1) for massage method) was significantly affected by capercaillie stimulation method. Sperm motility and morphology were not affected (P ≥ 0.05). Thus, for semen collection from captive kept capercaillie both methods can be used successfully. The dummy female can be an alternative to dorso-abdominal massage method, commonly used for semen collection from domesticated bird species.
人工授精（AI）对于解决在小型封闭鸟类种群中观察到的繁殖和生物多样性问题非常有帮助。使用AI成功生产受精卵取决于优质精液的采集。本研究比较了两种对圈养的黑琴鸡（Tetrao urogallus L.）进行雄性性刺激和精液采集的方法，黑琴鸡是欧洲最濒危的松鸡物种之一。通过使用雌性模型或采用背腹按摩法获取射精。记录了雄性对两种精液采集方法的个体反应差异以及精液质量差异。仅精子浓度（使用雌性模型时为432.4×10⁶ mL⁻¹，按摩法为614.5×10⁶ mL⁻¹）受黑琴鸡刺激方法的显著影响。精子活力和形态不受影响（P≥0.05）。因此，对于圈养黑琴鸡的精液采集，两种方法均可成功使用。雌性模型可作为通常用于从家养鸟类采集精液的背腹按摩法的替代方法。
