Singh Seema, Kumari Rina, Chakraborty Anirban, Hussain Sahid, Singh Manoj K, Das Prolay
Department of Chemistry, Indian Institute of Technology Patna, Patna 800013, Bihar, India.
Atomic and Molecular Physics Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
J Colloid Interface Sci. 2016 Jan 1;461:45-49. doi: 10.1016/j.jcis.2015.08.064. Epub 2015 Aug 28.
Formation of QD-array in solution phase guided by the self-assembly with DNA-melamine hybrid molecules is reported here. Melamine was conjugated with ssDNA using phosphoramidate chemistry. Aqueous soluble ZnSe/ZnS QDs conjugated to complementary ssDNA was self-assembled with the DNA-melamine hybrid molecules by DNA-hybridization. The self-assembly leads to the precise positioning of the QDs in QDs array where the inter QD distance is being maintained by the DNA sequence length. The QD array was characterized by gel electrophoresis, UV-visible and fluorescence spectrophotometry and circular dichroism. Direct visualization of the DNA-melamine hybrid molecule mediated QD array was made possible by atomic force microscopy (AFM) and transmission electron microscopy (TEM) analysis. Substantial increase in the fluorescence intensity and lifetime of the QDs was observed on array formation by DNA self-assembly.
本文报道了在溶液相中通过与DNA-三聚氰胺杂化分子自组装引导形成量子点阵列的过程。三聚氰胺通过氨基磷酸酯化学方法与单链DNA共轭。与互补单链DNA共轭的水溶性ZnSe/ZnS量子点通过DNA杂交与DNA-三聚氰胺杂化分子自组装。这种自组装导致量子点在量子点阵列中精确排列，其中量子点之间的距离由DNA序列长度维持。通过凝胶电泳、紫外可见分光光度法、荧光分光光度法和圆二色性对量子点阵列进行了表征。通过原子力显微镜（AFM）和透射电子显微镜（TEM）分析，可以直接观察到DNA-三聚氰胺杂化分子介导的量子点阵列。通过DNA自组装形成阵列时，观察到量子点的荧光强度和寿命大幅增加。
