Wang Jia-Zhong, Liu Yang, Wang Jin-Long, Lu Le, Zhang Ya-Fei, Lu Hong-Wei, Li Yi-Ming
Jia-Zhong Wang, Yang Liu, Jin-Long Wang, Le Lu, Ya-Fei Zhang, Hong-Wei Lu, Yi-Ming Li, Department of General Surgery, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710004, Shaanxi Province, China.
World J Gastroenterol. 2015 Sep 21;21(35):10113-25. doi: 10.3748/wjg.v21.i35.10113.
To study whether transfer of blood between the right gastroepiploic artery and gastroduodenal artery could lessens the damage to bile canaliculi.
Forty male Bama miniature pigs were divided into four groups as follows: a control group, two hepatic artery ischemia groups (1 h and 2 h), and a hepatic artery bridging group. The hemodynamics of the hepatic artery in the hepatic artery bridging group was measured using color Doppler ultrasound. Morphological changes in the bile canaliculus were observed by transmission electron microscopy. Cofilin, heat shock protein 27 and F-actin expression was detected by immunohistochemistry, Western blot, and real-time polymerase chain reaction. Terminal deoxynucleotidyl transferase-mediated nick end-labeling method was used to evaluate liver injury.
The hemodynamics was not changed in the hepatic artery bridging group. The microvilli in the bile canaliculus were impaired in the two hepatic artery ischemia groups. The down-regulation of cofilin and F-actin and up-regulation of heat shock protein 27 were observed in the two hepatic artery ischemia groups, while there were no significant differences between the control group and hepatic artery bridging group.
Hepatic artery ischemia aggravates damage to bile canaliculi, and this damage can be diminished by a hepatic artery bridging duct.
研究胃网膜右动脉与胃十二指肠动脉之间的血液转移是否能减轻对胆小管的损伤。
将40只雄性巴马小型猪分为四组：对照组、两个肝动脉缺血组（1小时和2小时）和一个肝动脉搭桥组。使用彩色多普勒超声测量肝动脉搭桥组肝动脉的血流动力学。通过透射电子显微镜观察胆小管的形态学变化。通过免疫组织化学、蛋白质免疫印迹法和实时聚合酶链反应检测丝切蛋白、热休克蛋白27和F-肌动蛋白的表达。采用末端脱氧核苷酸转移酶介导的缺口末端标记法评估肝损伤。
肝动脉搭桥组的血流动力学未发生改变。两个肝动脉缺血组的胆小管微绒毛受损。在两个肝动脉缺血组中观察到丝切蛋白和F-肌动蛋白下调以及热休克蛋白27上调，而对照组和肝动脉搭桥组之间无显著差异。
肝动脉缺血会加重对胆小管的损伤，而肝动脉搭桥导管可减轻这种损伤。
