[Rapid destroying of mRNAs in disaggregated Dictyostelium cells and effect of antibiotics].

Nogalamycin can inhibit RNA synthesis in both aggregated and disaggregated Dictyostelium discoideum cells. Cycloheximide and puromycin are effective in blocking translation. The late mRNAs, specific for the multicellular stage, degrade rapidly in disaggregated Dictyostelium cells. Nogalamycin or actinomycin D and daunomycin protect some of these mRNAs. Cycloheximide stabilizes all of the mRNAs, Puromycin, however, does not protect the mRNAs from destroying. These results imply that the rapid degradation of the late mRNAs during cell differentiating does not depend on nuclease or protein synthesis de novo.