阴茎鳞状细胞癌且人乳头瘤病毒呈阳性患者组织样本的蛋白质组学分析。
Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus.
作者信息
Koifman Leandro, Ornellas Paulo, Ornellas Antonio Augusto, Pereira Denise de Abreu, Zingali Benedeta Russolina, Cavalcanti Silvia Maria Baeta, Afonso Larissa Alves, Sandim Vanessa, Alves Gilda
机构信息
Serviço de Urologia, Hospital Municipal Souza Aguiar, Rio de Janeiro, Rio de Janeiro,Brasil.
Serviço de Urologia, Hospital Mário Kröeff, Rio de Janeiro, Rio de Janeiro, Brasil.
出版信息
Int Braz J Urol. 2015 Jul-Aug;41(4):642-54. doi: 10.1590/S1677-5538.IBJU.2014.0051.
PURPOSE
The aim of this study was to identify possible protein biomarkers and/or candidates for therapeutic targets in tissues of patients with SCCP, infected by HPV, applying one dimensional electrophoresis (1DE), followed by direct mass spectrometry (MS) analysis.
MATERIALS AND METHODS
Tissues from 10 HPV positive patients with SCCP and from 10 patients with HPV negative non-tumorous penile foreskins were analyzed applying 1D electrophoresis, followed by analysis with direct mass spectrometry (MS).
RESULTS
Sixty-three different proteins were identified in the first group and 50 in the second group. Recognition was possible for 28 proteins exclusively detected in Group 1 and 21 proteins presented only in Group 2.
CONCLUSION
Some proteins in the first group are directly involved in the development of other types of cancer, and therefore, suitable for analysis. Complement C3 protein is a strong candidate for evaluating SCCP patients.
目的
本研究的目的是通过一维电泳（1DE），随后进行直接质谱（MS）分析，在感染人乳头瘤病毒（HPV）的尖锐湿 疣癌前病变（SCCP）患者的组织中鉴定可能的蛋白质生物标志物和/或治疗靶点候选物。
材料与方法
对10例HPV阳性的SCCP患者的组织和10例HPV阴性的非肿瘤性阴茎包皮患者的组织进行一维电泳分析，随后进行直接质谱（MS）分析。
结果
第一组鉴定出63种不同的蛋白质，第二组鉴定出50种。仅在第一组中检测到的28种蛋白质和仅在第二组中出现的21种蛋白质可以被识别。
结论
第一组中的一些蛋白质直接参与其他类型癌症的发展，因此适合进行分析。补体C3蛋白是评估SCCP患者的有力候选物。
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