Khosravanian Hajar, Razi Mazdak, Farokhi Farah, Khosravanian Narges
Department of Biology, Faculty of Basic Science, Urmia University, Urmia, Iran.
Department of Comparative Histology & Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Int Braz J Urol. 2015 Jul-Aug;41(4):773-90. doi: 10.1590/S1677-5538.IBJU.2013.0148.
This study aimed to investigate the protective effects of isolated and co-administration of vitamin E (VitE) and dexamethasone (DEX) on varicocele (VCL)-induced damages in testicular tissue.
Wistar rats were divided into five groups (n=6), including; control-sham, non-treated VCL-induced, VitE-treated VCL-induced (VitE, 150 mg/kg, orally), DEX-administrated VCL-induced (DEX, 0.125 mg/kg, i.p.), VitE+DEX-received VCL-induced animals. The antioxidant status analyses, histopathological examinations, hormonal assay and tissue levels of alkaline phosphatase (ALP) were analyzed. The germinal epithelium RNA damage and Leydig cells steroidogenesis were analyzed. Moreover, the Hsp70-2 protein expression was examined based on immunohistochemical and western blot analyses. The sperm parameters, DNA integrity and chromatin condensation were investigated.
VitE and DEX in simultaneous form of administration significantly (P<0.05) down-regulated the tissue ALP level and attenuated the VCL-decreased GSH-px, SOD and TAC levels and remarkably (P<0.05) down-regulated the testicular malondialdehyde (MDA) and nitric oxide (NO) contents. The VCL-induced histopathological alterations significantly (P<0.05) improved in VitE and DEX-administrated animals. The VitE and DEX co-administration reduced the VCL-increased RNA damage and elevated the Leydig cells steroidogenic activity. The Hsp70-2 protein level completely (P<0.05) increased in VitE and DEX alone-and-simultaneous-administrated animals. Finally, the VitE and DEX could significantly (P<0.05) improve the VCL-decreased semen quality and improved the sperm DNA integrity and chromatin condensation.
Our data suggest that Vit E by up-regulating the antioxidant status and DEX by reducing inflammation-dependent oxidative and nitrosative stresses could improve the VCL-reduced Hsp70-2 chaperone expression and ultimately protected the testicular endocrine activities and promoted the spermatogenesis process.
本研究旨在探讨单独及联合给予维生素E（VitE）和地塞米松（DEX）对精索静脉曲张（VCL）所致睾丸组织损伤的保护作用。
将Wistar大鼠分为五组（n = 6），包括：对照 - 假手术组、未治疗的VCL诱导组、VitE治疗的VCL诱导组（VitE，150 mg/kg，口服）、DEX给药的VCL诱导组（DEX，0.125 mg/kg，腹腔注射）、接受VitE + DEX的VCL诱导组动物。分析抗氧化状态、组织病理学检查、激素测定及碱性磷酸酶（ALP）的组织水平。分析生精上皮RNA损伤和睾丸间质细胞类固醇生成。此外，基于免疫组织化学和蛋白质印迹分析检测Hsp70 - 2蛋白表达。研究精子参数、DNA完整性和染色质凝聚情况。
同时给予VitE和DEX可显著（P < 0.05）下调组织ALP水平，减轻VCL导致的谷胱甘肽过氧化物酶（GSH - px）、超氧化物歧化酶（SOD）和总抗氧化能力（TAC）水平降低，并显著（P < 0.05）下调睾丸丙二醛（MDA）和一氧化氮（NO）含量。在给予VitE和DEX的动物中，VCL诱导的组织病理学改变显著（P < 0.05）改善。VitE和DEX联合给药减少了VCL增加的RNA损伤，提高了睾丸间质细胞类固醇生成活性。单独及同时给予VitE和DEX的动物中，Hsp70 - 2蛋白水平完全（P < 0.05）升高。最后，VitE和DEX可显著（P < 0.05）改善VCL降低的精液质量，改善精子DNA完整性和染色质凝聚。
我们的数据表明，VitE通过上调抗氧化状态，DEX通过减轻炎症依赖性氧化和亚硝化应激，可改善VCL降低的Hsp70 - 2伴侣蛋白表达，最终保护睾丸内分泌活动并促进精子发生过程。
