Mazhari Sara, Razi Mazdak, Sadrkhanlou Rajabali
Division of Histology and Embryology, Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, P.O. Box: 1177, Urmia, Iran.
Int Urol Nephrol. 2018 Jun;50(6):1039-1052. doi: 10.1007/s11255-018-1862-5. Epub 2018 Apr 5.
The present study was done to investigate the ameliorative effect of silymarin (SMN) and celecoxib (CEL) on varicocele (VCL)-induced detrimental impact in testicular tissue.
Mature Wistar rats were divided into control and test groups. Following VCL induction, the animals in test group were subdivided into non-treated VCL-induced, SMN-treated (50 mg/kg, orally), CEL-treated (10 mg/kg) and SMN + CEL-treated groups. Following 60 days, testicular total antioxidant capacity (TAC), malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GSH-px), total thiol molecules (TTM), mRNA and protein levels of COX2 and mRNA level of iNos were analyzed. Moreover, the germinal cells apoptosis and mRNA damage were examined.
Observations revealed that co-administration of SMN and CEL significantly (P < 0.05) up-regulated TAC, SOD, GSH-px and TTM levels and resulted in a remarkable (P < 0.05) reduction in iNos and COX2 expression, NO and MDA contents. The animals in SMN + CEL-treated group exhibited significantly (P < 0.05) lower number of apoptotic cells and cells with mRNA damage per one mm.
The SMN by up-regulating testicular TAC, SOD, GSH-px and TTM levels and the CEL by inhibiting COX2 and iNos expression as well as NO content could fairly ameliorate the VCL-decreased spermatogenesis.
本研究旨在探讨水飞蓟素（SMN）和塞来昔布（CEL）对精索静脉曲张（VCL）所致睾丸组织有害影响的改善作用。
将成年Wistar大鼠分为对照组和试验组。诱导VCL形成后，将试验组动物再细分为未治疗的VCL诱导组、SMN治疗组（50毫克/千克，口服）、CEL治疗组（10毫克/千克）和SMN + CEL治疗组。60天后，分析睾丸总抗氧化能力（TAC）、丙二醛（MDA）、一氧化氮（NO）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-px）、总硫醇分子（TTM）、COX2的mRNA和蛋白水平以及iNos的mRNA水平。此外，检测生精细胞凋亡和mRNA损伤情况。
观察结果显示，SMN和CEL联合使用显著（P < 0.05）上调了TAC、SOD、GSH-px和TTM水平，并导致iNos和COX2表达、NO和MDA含量显著（P < 0.05）降低。SMN + CEL治疗组动物每毫米的凋亡细胞和mRNA损伤细胞数量显著（P < 0.05）减少。
SMN通过上调睾丸TAC、SOD、GSH-px和TTM水平，CEL通过抑制COX2和iNos表达以及NO含量，可相当程度地改善VCL导致的精子发生减少。
