视网膜色素上皮细胞收缩特性的研究。

Investigations on contractile properties of retinal pigment epithelial cells.

作者信息

Yamakawa R, Sorgente N, Ryan S J

机构信息

Department of Ophthalmology, Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles.

出版信息

Ophthalmologica. 1989;199(4):165-72. doi: 10.1159/000310036.

DOI:10.1159/000310036

PMID:2640294

Abstract

We have investigated the contractile properties of human and bovine retinal pigment epithelial (RPE) cells in culture. In collagen gels, RPE cells sent out processes which were able to retract the gels. In a glycerinated model of contraction, RPE cells contracted and aggregated into clusters in response to the addition of ATP to the media. This contraction was very likely mediated by contractile proteins which are arranged in circumferential microfilament bundles in RPE cells. SDS-PAGE analysis of the RPE cell cytoskeletal elements showed protein bands of 200 kD and 43 kD, probably heavy-chain myosin and actin, respectively; in human RPE, cells bands of 58 kD and 52 kd, representing intermediate filament subunits, were evident; in bovine cells the 58-kD band was observed.

摘要

我们研究了培养的人及牛视网膜色素上皮（RPE）细胞的收缩特性。在胶原凝胶中，RPE细胞伸出能够使凝胶收缩的突起。在甘油化收缩模型中，向培养基中添加ATP后，RPE细胞收缩并聚集成簇。这种收缩很可能是由RPE细胞中沿圆周微丝束排列的收缩蛋白介导的。对RPE细胞细胞骨架成分的SDS-PAGE分析显示，有200 kD和43 kD的蛋白条带，可能分别为重链肌球蛋白和肌动蛋白；在人RPE细胞中，代表中间丝亚基的58 kD和52 kD的条带很明显；在牛细胞中观察到了58-kD的条带。