Szafrańska B, Ziecik A
Institute of Animal Physiology, University of Agriculture and Technology, Ołsztyn-Kortowo, Poland.
Acta Physiol Hung. 1989;74(3-4):253-8.
Active immunization of four adult pigs with highly purified porcine luteinizing hormone (pLH)--using method of multiside intradermal injections--has been performed and resulted in the production of specific antibodies. Immunization caused prolongation of estrous cycle to 47-49 days in two gilts and to 26 days in the other ones. Obtained anti-pLH pig serum was administered intravenously to 40 day pregnant gilt during 5 days (10 ml of serum, twice daily). Blood plasma progesterone (P4) concentrations decreased significantly from 8-13 to 2-4 ng/ml after two days of infusion and remained at this level for the next 5 days. Administration of this anti-pLH pig serum to gilt in the luteal phase of the estrous cycle caused the inhibition P4 to undetectable amounts. The different results were found after the passive immunization of 40 day pregnant gilt with rabbit anti-pLH globulin preparation (5 days, equivalent to 3 ml of original undiluted serum, twice daily). Although after two days of infusion P4 concentration decreased, in the next days P4 level slowly increased to pretreatment concentrations. The data suggest the possibility of specific anti-pLH antibody production in pigs by using active immunization, and the repeated utilization of such obtained antiserum in the same species for the inhibition of corpus luteum (CL) function.
采用多部位皮内注射法，用高度纯化的猪促黄体激素（pLH）对4头成年母猪进行主动免疫，成功产生了特异性抗体。免疫导致两头后备母猪的发情周期延长至47 - 49天，另外两头延长至26天。将获得的抗pLH猪血清在5天内（每天两次，每次10 ml）静脉注射给怀孕40天的后备母猪。输注两天后，血浆孕酮（P4）浓度从8 - 13 ng/ml显著降至2 - 4 ng/ml，并在接下来的5天维持在该水平。在发情周期的黄体期将这种抗pLH猪血清注射给后备母猪，导致P4抑制至无法检测的水平。用兔抗pLH球蛋白制剂对怀孕40天的后备母猪进行被动免疫（5天，相当于每天两次，每次3 ml未稀释的原始血清）后，结果有所不同。虽然输注两天后P4浓度下降，但在接下来的几天里，P4水平缓慢回升至预处理浓度。数据表明通过主动免疫在猪体内产生特异性抗pLH抗体的可能性，以及在同一物种中重复使用这种获得的抗血清来抑制黄体（CL）功能的可能性。
