利用5'-cDNA末端快速扩增技术（5'-RACE）精确鉴定细菌全基因组转录起始位点

Precise Identification of Genome-Wide Transcription Start Sites in Bacteria by 5'-Rapid Amplification of cDNA Ends (5'-RACE).

作者信息

Matteau Dominick, Rodrigue Sébastien

机构信息

Département de biologie, Faculté des sciences, Université de Sherbrooke, 2500 boulevard de l'Université, Sherbrooke, QC, Canada, J1K 2R1.

出版信息

Methods Mol Biol. 2015;1334:143-59. doi: 10.1007/978-1-4939-2877-4_9.

DOI:10.1007/978-1-4939-2877-4_9

PMID:26404148

Abstract

Transcription start sites are commonly used to locate promoter elements in bacterial genomes. TSS were previously studied one gene at a time, often through 5'-rapid amplification of cDNA ends (5'-RACE). This technique has now been adapted for high-throughput sequencing and can be used to precisely identify TSS in a genome-wide fashion for practically any bacterium, which greatly contributes to our understanding of gene regulatory networks in microorganisms.

摘要

转录起始位点常用于定位细菌基因组中的启动子元件。以前对转录起始位点的研究通常是一次针对一个基因，常常通过5'-cDNA末端快速扩增（5'-RACE）技术来进行。现在这项技术已适用于高通量测序，可用于以全基因组方式精确鉴定几乎任何一种细菌的转录起始位点，这极大地促进了我们对微生物基因调控网络的理解。