印度旁遮普邦地区微小扇头蜱（蜱螨亚纲：硬蜱科）田间种群对伊维菌素产生抗性的首次报告。
First report of ivermectin resistance in field populations of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in Punjab districts of India.
作者信息
Singh Nirbhay Kumar, Singh Harkirat, Prerna Mranalini, Rath Shitanshu S
机构信息
Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, India.
Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, India.
出版信息
Vet Parasitol. 2015 Nov 30;214(1-2):192-4. doi: 10.1016/j.vetpar.2015.09.014. Epub 2015 Sep 14.
The larval immersion test (LIT) was used on the progenies of Rhipicephalus (Boophilus) microplus collected from four districts of the Punjab state, India to test the resistance to ivermectin. The regression graphs of probit mortality of larval ticks plotted against log values of increasing concentrations of ivermectin were utilized for the determination of slope of mortality, lethal concentration for 50%(LC50), 95%(LC95), resistance ratios (RR50, RR95) and the resistance levels (RL). Values of the coefficient of determination (R(2)) for LIT ranged from 0.900 to 0.978, and the RR50 and RR95 values against ivermectin ranged from 2.97 to 8.85 and 2.42 to 8.47, respectively, indicating resistance status in all field isolates. On the basis of RR values, three field isolates (BAT, GUR, HOS) showed level II, whereas PTK isolate showed presence of level I resistance status against ivermectin. This appears to be the first report of ivermectin resistance in R. (B.) microplus from Punjab, India.
对从印度旁遮普邦四个地区采集的微小牛蜱后代进行了幼虫浸泡试验（LIT），以测试其对伊维菌素的抗性。利用幼虫蜱虫的概率死亡率回归图，以伊维菌素浓度增加的对数值作图，来确定死亡率斜率、50%致死浓度（LC50）、95%致死浓度（LC95）、抗性比率（RR50、RR95）和抗性水平（RL）。LIT的决定系数（R(2)）值范围为0.900至0.978，对伊维菌素的RR50和RR95值分别为2.97至8.85和2.42至8.47，表明所有野外分离株均存在抗性状态。根据RR值，三个野外分离株（BAT、GUR、HOS）表现为II级抗性，而PTK分离株对伊维菌素表现为I级抗性状态。这似乎是印度旁遮普邦微小牛蜱对伊维菌素抗性的首次报道。
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