一种用于简化质谱仪器且灵敏度增强的脉冲针孔大气压接口。
A pulsed pinhole atmospheric pressure interface for simplified mass spectrometry instrumentation with enhanced sensitivity.
作者信息
Wei Yongzheng, Bian Cunjuan, Ouyang Zheng, Xu Wei
机构信息
School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
Biomedical Engineering Department, Purdue University, West Lafayette, IN, 47907, USA.
出版信息
Rapid Commun Mass Spectrom. 2015 Apr 30;29(8):701-6. doi: 10.1002/rcm.7140.
RATIONALE
A proof-of-concept pulsed pinhole atmospheric pressure interface, namely PP-API, was developed and characterized for mass spectrometry instrumentation.
METHODS
The PP-API was analyzed and optimized theoretically with respect to gas flow rate, opening force and dimensions. A PP-API interfaced mass spectrometry system was then constructed and tested.
RESULTS
As a discontinuous pressure interface, the PP-API allows efficient ion transfer from atmosphere pressure ionization sources to ion traps directly. Both nano-ESI and APCI ionization sources have been successfully integrated with the PP-API interface for the detection of volatile, organic, peptide and polymer samples. The use of multiple ionization sources has also been demonstrated to enhance signal intensity, as well as avoid charge competitions in ionization sources.
CONCLUSIONS
With simplified geometry and high ion transfer efficiency, this PP-API would enable miniaturized mass spectrometry systems with high sensitivity.
原理
开发了一种概念验证型脉冲针孔大气压接口，即PP - API，并对其进行了质谱仪器表征。
方法
对PP - API在气体流速、开启力和尺寸方面进行了理论分析和优化。然后构建并测试了一个与PP - API相连的质谱系统。
结果
作为一种间断压力接口，PP - API可实现从大气压电离源到离子阱的高效离子传输。纳升电喷雾电离（nano - ESI）和大气压化学电离（APCI）电离源均已成功与PP - API接口集成，用于检测挥发性、有机、肽和聚合物样品。还证明了使用多种电离源可增强信号强度，并避免电离源中的电荷竞争。
结论
这种PP - API具有简化的几何结构和高离子传输效率，将能够实现高灵敏度的小型化质谱系统。
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