Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry 605 014, India.
Centre for Bioinformatics, Pondicherry University, Puducherry 605 014, India.
J Photochem Photobiol B. 2015 Dec;153:137-44. doi: 10.1016/j.jphotobiol.2015.09.013. Epub 2015 Sep 15.
The studies on the interaction between tRNA (transfer RNA) and small molecules are an area of remarkable recent attention. For this notion a fundamental knowledge of the molecular features involving the interaction of small molecules with tRNA is crucial. Hence, in the present study we have investigated the interaction of TXER (troxerutin), natural bioflavonoid rutin derivative with yeast tRNA by using various spectroscopic techniques and molecular docking studies. The UV absorption and fluorescence emission studies demonstrated external binding of TXER on tRNA with low binding constant values as compared to strong binders. Circular dichroism (CD) spectroscopy study revealed that TXER did not show any significant modification on native conformation of tRNA. Furthermore in electrochemical study, the complex of TXER-tRNA did not expose any noticeable positive potential peak shift which indicated an interaction of TXER with tRNA by electrostatic or external binding mode. The docking study showed that the hydrogen and hydrophobic interactions were involved in binding of TXER-tRNA with docking score -7.0 kcal/mol. These findings led us to confirm the interaction of TXER on tRNA through external binding with low binding affinity, indicating its potential bioapplication in the future.
研究 tRNA（转移 RNA）与小分子之间的相互作用是当前备受关注的领域。为了实现这一目标，深入了解小分子与 tRNA 相互作用所涉及的分子特征至关重要。因此，在本研究中，我们采用多种光谱技术和分子对接研究方法，研究了天然生物类黄酮芦丁衍生物 TXER（曲克芦丁）与酵母 tRNA 的相互作用。紫外吸收和荧光发射研究表明，TXER 与 tRNA 的外部结合具有较低的结合常数值，低于强结合物。圆二色性（CD）光谱研究表明，TXER 对 tRNA 的天然构象没有明显的修饰。此外，在电化学研究中，TXER-tRNA 复合物没有显示出任何明显的正电势峰位移，这表明 TXER 与 tRNA 之间的相互作用通过静电或外部结合模式发生。对接研究表明，TXER-tRNA 的结合涉及氢键和疏水相互作用，对接评分为-7.0 kcal/mol。这些发现使我们能够通过低亲和力的外部结合来确认 TXER 与 tRNA 的相互作用，表明其在未来具有潜在的生物应用价值。
