Notch信号通路在增殖性玻璃体视网膜病变发展过程中调节M2型巨噬细胞极化。
Notch signaling regulates M2 type macrophage polarization during the development of proliferative vitreoretinopathy.
作者信息
Zhang Jingjing, Zhou Qingjun, Yuan Gongqiang, Dong Muchen, Shi Weiyun
机构信息
Shandong Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Jinan, China.
State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.
出版信息
Cell Immunol. 2015 Nov-Dec;298(1-2):77-82. doi: 10.1016/j.cellimm.2015.09.005. Epub 2015 Sep 16.
Macrophages play an important role in the pathogenesis of proliferative vitreoretinopathy (PVR). M2 macrophages can promote tissue remodeling and repair. In this study, CD206 positive M2 type macrophages were found in preretinal fibrous membranes of the mouse model of PVR induced by the intravitreal injection of retinal pigment epithelial (RPE) cells. Notch signaling determines M2 macrophage polarization. The specific inhibition of Notch signaling pathway by the intravitreal injection of γ-secretase inhibitor DAPT attenuated RPE cells-induced PVR formation as demonstrated by the decreased expression of α-SMA, and inhibited M2 type macrophage infiltation as demonstrated by the decreased expression of Arg-1. Notch signaling may modulate PVR formation by regulating M2 type macrophage polarization.
巨噬细胞在增殖性玻璃体视网膜病变（PVR）的发病机制中起重要作用。M2巨噬细胞可促进组织重塑和修复。在本研究中，在通过玻璃体内注射视网膜色素上皮（RPE）细胞诱导的PVR小鼠模型的视网膜前纤维膜中发现了CD206阳性M2型巨噬细胞。Notch信号决定M2巨噬细胞极化。玻璃体内注射γ-分泌酶抑制剂DAPT对Notch信号通路的特异性抑制减弱了RPE细胞诱导的PVR形成，α-SMA表达降低证明了这一点，并且抑制了M2型巨噬细胞浸润，Arg-1表达降低证明了这一点。Notch信号可能通过调节M2型巨噬细胞极化来调节PVR形成。
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