Sotgia Salvatore, Zinellu Angelo, Arru Dionigia, Nieddu Stefano, Strina Alessandro, Ariu Federica, Pintus Gianfranco, Carru Ciriaco, Bogliolo Luisa, Ledda Sergio
Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
Res Vet Sci. 2015 Oct;102:238-41. doi: 10.1016/j.rvsc.2015.09.003. Epub 2015 Sep 6.
L-ergothioneine levels were measured in amniotic fluid of pregnant sheep after natural mating and transfer of vitrified/thawed in-vitro produced embryos. Amniotic fluids were collected between 60 and 65 and 80-85 days of gestation and analysed by an ultra-performance liquid chromatographic (UPLC)method with fluorescence detection. L-Ergothioneine concentrations ranged between 0.23 and 9.36 μmol/L and were significantly higher in pregnancy obtained by the transfer of vitrified/thawed in-vitro produced embryos. Conversely, no significant changes in amniotic fluid L-ergothioneine concentrations were observed according to the stages of pregnancy considered in this study. These findings suggest that L-ergothioneine concentrations, are not affected as much by the gestational age, but rather by the method used to induce the pregnancy. On the whole, the measurement of L-ergothioneine in amniotic fluid could serve as a useful biomarker of oxidative stress and/or inflammatory state in pregnancy.
在自然交配以及移植经玻璃化冷冻/解冻的体外生产胚胎后，对怀孕绵羊的羊水进行了L-麦角硫因水平测定。在妊娠60至65天以及80至85天之间收集羊水，并采用超高效液相色谱（UPLC）荧光检测法进行分析。L-麦角硫因浓度在0.23至9.36μmol/L之间，通过移植经玻璃化冷冻/解冻的体外生产胚胎所获得的妊娠中，其浓度显著更高。相反，根据本研究中所考虑的妊娠阶段，未观察到羊水L-麦角硫因浓度有显著变化。这些发现表明，L-麦角硫因浓度受胎龄的影响不大，而更多地受诱导妊娠所用方法的影响。总体而言，测定羊水中的L-麦角硫因可作为孕期氧化应激和/或炎症状态的有用生物标志物。
