Yuan Y, Chen S, Gleber S C, Lai B, Brister K, Flachenecker C, Wanzer B, Paunesku T, Vogt S, Woloschak G E
Department of Radiation Oncology, Northwestern University, Chicago, IL 60611, USA.
X-ray Sciences Division, Argonne National Laboratory, Argonne, IL 60439, USA.
J Phys Conf Ser. 2013;463. doi: 10.1088/1742-6596/463/1/012020.
The targeted delivery of FeO@TiO2 nanoparticles to cancer cells is an important step in their development as nanomedicines. We have synthesized nanoparticles that can bind the Epidermal Growth Factor Receptor, a cell surface protein that is overexpressed in many epithelial type cancers. In order to study the subcellular distribution of these nanoparticles, we have utilized the sub-micron resolution of X-ray Fluorescence Microscopy to map the locationof FeO4@TiO NPs and other trace metal elements within HeLa cervical cancer cells. Here we demonstrate how the higher resolution of the newly installed Bionanoprobe at the Advanced Photon Source at Argonne National Laboratory can greatly improve our ability to distinguish intracellular nanoparticles and their spatial relationship with subcellular compartments.
将FeO@TiO2纳米颗粒靶向递送至癌细胞是其作为纳米药物发展的重要一步。我们合成了能够结合表皮生长因子受体的纳米颗粒，该受体是一种在许多上皮型癌症中过度表达的细胞表面蛋白。为了研究这些纳米颗粒的亚细胞分布，我们利用X射线荧光显微镜的亚微米分辨率来绘制FeO4@TiO NPs和其他痕量金属元素在HeLa宫颈癌细胞内的位置。在此，我们展示了阿贡国家实验室先进光子源新安装的生物纳米探针的更高分辨率如何能极大地提高我们区分细胞内纳米颗粒及其与亚细胞区室空间关系的能力。
