Lee Si Hyung, Kong Yoon Jin, Lyu Jungmook, Lee Heuiran, Park Keerang, Park Tae Kwann
1 Department of Ophthalmology, Severance Hospital, Institute of Vision Research, Yonsei University College of Medicine , Seoul, South Korea.
2 Department of Ophthalmology, Soonchunhyang University College of Medicine , Bucheon Hospital, Bucheon, South Korea.
Hum Gene Ther Methods. 2015 Oct;26(5):159-61. doi: 10.1089/hgtb.2015.102.
Retinal transduction by intravitreally administered adeno-associated viral (AAV) vector is previously known to be extremely limited to the neural retina except AAV2 capsid type. Recently, we showed that prior laser photocoagulation enhances retinal transduction of intravitreally administered AAV vectors, including the outer retina and retinal pigment epithelium (RPE). Here, by performing short-pulse laser pretreatment on the mouse retina, we demonstrate RPE cells transduced by three different capsid types of AAV vectors, AAV2, AAV5, and AAV8, using RPE wholemounts. For all capsid types, laser pretreatment effectively induced the transduction of RPE cells in and around the laser site.
除了AAV2衣壳类型外，先前已知玻璃体内注射腺相关病毒（AAV）载体介导的视网膜转导极其局限于神经视网膜。最近，我们发现，预先进行激光光凝可增强玻璃体内注射的AAV载体在视网膜的转导，包括外视网膜和视网膜色素上皮（RPE）。在此，通过对小鼠视网膜进行短脉冲激光预处理，我们使用RPE整装片证明了三种不同衣壳类型的AAV载体（AAV2、AAV5和AAV8）可转导RPE细胞。对于所有衣壳类型，激光预处理均有效地诱导了激光部位及其周围RPE细胞的转导。
