Kitou Yoshiyuki, Nakajima Yuichi, Maeda Kazuyuki, Jin Qi, Nishiuchi Takumi, Kanamaru Kyoko, Kobayashi Tetsuo, Kimura Makoto
a Department of Biological Mechanisms and Functions , Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya , Japan.
b Division of Functional Genomics, Advanced Science Research Centre , Kanazawa University , Kanazawa , Japan.
Biosci Biotechnol Biochem. 2016;80(2):414-7. doi: 10.1080/09168451.2015.1088374. Epub 2015 Sep 28.
Disruption of two Fusarium genes that negatively regulate trichothecene biosynthesis was reported to cause a drastic increase in trichothecene production. However, careful inspection of these genes revealed that neither was significantly related to trichothecene production. Agmatine medium maintained the expression of trichothecene genes at significant levels, resulting in a 2-3-fold increase in the final yield, as compared to glutamine medium.
据报道，两个负调控单端孢霉烯生物合成的镰刀菌基因的破坏会导致单端孢霉烯产量急剧增加。然而，对这些基因的仔细检查发现，它们与单端孢霉烯产量均无显著关联。与谷氨酰胺培养基相比，胍丁胺培养基能使单端孢霉烯基因的表达维持在显著水平，最终产量提高了2至3倍。
