Wattanathorn Jintanaporn, Sutalangka Chatchada
1 Department of Physiology, Faculty of Medicine, Khon Kaen University , Khon Kaen, Thailand .
2 Integrative Complementary Alternative Medicine Research and Development Center, Khon Kaen University , Khon Kaen, Thailand .
Rejuvenation Res. 2016 Aug;19(4):273-85. doi: 10.1089/rej.2015.1729. Epub 2016 Feb 23.
Based on pivotal roles of oxidative stress, dopaminergic and cholinergic systems on the pathophysiology of Parkinson's disease (PD), the searching for functional food for patients attacked with PD from Cyperus rotundus and Zingiber officinale, the substances possessing antioxidant activity, and the suppression effects on monoamine oxidase B (MAO-B) and acetylcholinesterase (AChE) have been considered. In this study, we aimed to determine the effect of the combined extract of C. rotundus and Z. officinale (CP1) to improve motor and memory deficits, neurodegeneration, oxidative stress, and functions of both cholinergic and dopaminergic systems in the animal model of PD induced by 6-hydroxydopamine hydrochloride (6-OHDA). Male Wistar rats, weighing 180-220 g, were induced unilateral lesion at right substantia nigra by 6-OHDA and were orally given CP1 at doses of 100, 200, and 300 mg/kg body weight for 14 days after 6-OHDA injection. The results showed that the 6-OHDA rats treated with CP1 increased spatial memory, but decreased neurodegeneration, malondialdehyde level, and AChE activity in hippocampus. The decreased motor disorder and neurodegeneration in substantia nigra together with the enhanced catalase activity, but decreased MAO-B activity in striatum, were also observed. The memory enhancing effect of CP1 might occur through the improved oxidative stress and the enhanced cholinergic function, whereas the effect to improve motor disorder of CP1 might occur through the enhanced dopaminergic function in striatum by decreasing the degeneration of dopaminergic neurons and the suppression of MAO-B. Therefore, CP1 is the potential functional food against PD. However, further researches in clinical trial and drug interactions are essential.
基于氧化应激、多巴胺能和胆碱能系统在帕金森病（PD）病理生理学中的关键作用，人们考虑从具有抗氧化活性以及对单胺氧化酶B（MAO - B）和乙酰胆碱酯酶（AChE）具有抑制作用的香附（Cyperus rotundus）和生姜（Zingiber officinale）中寻找用于PD患者的功能性食品。在本研究中，我们旨在确定香附与生姜的联合提取物（CP1）对改善盐酸6 - 羟基多巴胺（6 - OHDA）诱导的PD动物模型中的运动和记忆缺陷、神经退行性变、氧化应激以及胆碱能和多巴胺能系统功能的影响。体重为180 - 220 g的雄性Wistar大鼠通过6 - OHDA诱导右侧黑质单侧损伤，并在6 - OHDA注射后以100、200和300 mg/kg体重的剂量口服给予CP1，持续14天。结果表明，用CP1处理的6 - OHDA大鼠空间记忆增强，但海马中的神经退行性变、丙二醛水平和AChE活性降低。还观察到黑质中运动障碍和神经退行性变减少，同时纹状体中的过氧化氢酶活性增强，但MAO - B活性降低。CP1的记忆增强作用可能通过改善氧化应激和增强胆碱能功能而发生，而CP1改善运动障碍的作用可能通过减少多巴胺能神经元的变性和抑制MAO - B来增强纹状体中的多巴胺能功能而实现。因此，CP1是一种潜在的抗PD功能性食品。然而，在临床试验和药物相互作用方面的进一步研究至关重要。
