Hu Xiangquan, Chai Jie, Liu Yanfei, Liu Bin, Yang Binsheng
Institute of Molecular Science, Key Laboratory of Chemical Biology of Molecular Engineering of Education Ministry, Shanxi University, Taiyuan 030006, China.
Institute of Molecular Science, Key Laboratory of Chemical Biology of Molecular Engineering of Education Ministry, Shanxi University, Taiyuan 030006, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Jan 15;153:505-9. doi: 10.1016/j.saa.2015.09.008. Epub 2015 Sep 25.
Cellular uptake of Cr(VI), followed by its reduction to Cr(III) with the formation of kinetically inert Cr(III) complexes, is a complex process. To better understand its physiological and pathological functions, efficient methods for the monitoring of Cr(VI) are desired. In this paper a selective fluorescent probe L, rhodamine hydrazide bearing a benzo[b]furan-2-carboxaldehyde group, was demonstrated as a red chemosensor for Cr(III) at about 586 nm. This probe has been used to probe Cr(III) which is reduced from Cr(VI) by reductants such as glutathione (GSH), vitamin C, cysteine (Cys), H2O2 and Dithiothreitol (DTT) by fluorescence spectra. Cr(VI) metabolism in vivo is primarily driven by Vc and GSH. Vc could reduce CrO4(2-) to Cr(III) in a faster rate than GSH. The indirectly detection limit for Cr(VI) by L+GSH system was determined to be 0.06 μM at pH=6.2. Moreover, the confocal microscopy image experiments indicated that Cr(VI) can be reduced to Cr(III) inside cells rapidly and the resulted Cr(III) can be captured and imaged timely by L.
细胞对六价铬的摄取，随后将其还原为三价铬并形成动力学惰性的三价铬配合物，是一个复杂的过程。为了更好地理解其生理和病理功能，需要有效的六价铬监测方法。本文展示了一种选择性荧光探针L，即带有苯并[b]呋喃-2-甲醛基团的罗丹明酰肼，作为一种在约586nm处对三价铬的红色化学传感器。该探针已用于通过荧光光谱探测由谷胱甘肽（GSH）、维生素C、半胱氨酸（Cys）、过氧化氢（H2O2）和二硫苏糖醇（DTT）等还原剂将六价铬还原得到的三价铬。体内六价铬的代谢主要由维生素C和谷胱甘肽驱动。维生素C比谷胱甘肽能更快地将铬酸根离子（CrO4(2-)）还原为三价铬。在pH = 6.2时，L + GSH系统对六价铬的间接检测限被确定为0.06μM。此外，共聚焦显微镜图像实验表明，六价铬能在细胞内迅速还原为三价铬，并且生成的三价铬能被L及时捕获并成像。
