微小RNA-23a调控3T3-L1脂肪细胞分化。

MicroRNA-23a regulates 3T3-L1 adipocyte differentiation.

作者信息

Shen Linyuan, Zhang Yi, Du Jingjing, Chen Li, Luo Jia, Li Xuewei, Li Mingzhou, Tang Guoqing, Zhang Shunhua, Zhu Li

机构信息

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

Department of Animal Science, Xichang College, Xichang 615000, China.

出版信息

Gene. 2016 Jan 10;575(2 Pt 3):761-4. doi: 10.1016/j.gene.2015.09.060. Epub 2015 Sep 28.

DOI:10.1016/j.gene.2015.09.060

PMID:26415879

Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs, which are involved in regulation of a variety of biological processes. Since previous studies regarding the role of miRNAs in the regulation of adipogenic differentiation have shown that miRNA-27a, one member of miRNA-23a∼27a∼24 cluster, could suppress adipogenesis. We now investigated whether miRNA-23a regulates adipogenic differentiation. In the present study, we showed that the expression of miRNA-23a is decreased during the process of adipogenic differentiation. Over-expression of miRNA-23a decreased lipid accumulation and triglyceride content in 3T3-L1 adipocytes. Our results also demonstrated that miRNA-23a decreases mRNA levels of adipocyte-specific genes involved in lipogenic transcription, fatty acid synthesis and fatty acid transport. These findings suggested miRNA-23a to be a new type of adipogenic depressor and to play an important role in regulating adipocyte differentiation.

摘要

微小RNA（miRNA）是一类小的非编码RNA，参与多种生物学过程的调控。由于先前关于miRNA在脂肪生成分化调控中作用的研究表明，miRNA-23a∼27a∼24簇的成员之一miRNA-27a可抑制脂肪生成。我们现在研究了miRNA-23a是否调节脂肪生成分化。在本研究中，我们发现miRNA-23a在脂肪生成分化过程中表达降低。miRNA-23a的过表达减少了3T3-L1脂肪细胞中的脂质积累和甘油三酯含量。我们的结果还表明，miRNA-23a降低了参与脂肪生成转录、脂肪酸合成和脂肪酸转运的脂肪细胞特异性基因的mRNA水平。这些发现表明miRNA-23a是一种新型的脂肪生成抑制因子，在调节脂肪细胞分化中起重要作用。