Wang Ming-qing, Chi Heng, Li Mo-fei
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Fish Shellfish Immunol. 2015 Nov;47(1):461-9. doi: 10.1016/j.fsi.2015.09.036. Epub 2015 Sep 28.
Chemokines are a large family of chemotactic cytokines. Based on the arrangement of the first two cysteine residues, chemokines are divided into four groups, one of which is the CC chemokine group. In this study, we characterized a CC chemokine, CsCCL21, from half-smooth tongue sole (Cynoglossus semilaevis), and analyzed its activity. CsCCL21 contains two conserved N-terminal cysteine residues in a NCCL motif and is phylogenetically related to the CCL19/21/25 subgroup of CC chemokines. CsCCL21 was constitutively expressed in nine tissues and significantly upregulated by bacterial and viral infection. The recombinant CsCCL21 (rCsCCL21) induced migration of peripheral blood leukocytes. When the two conserved cysteine residues in the NCCL motif were mutated, the chemotactic activity of rCsCCL21 was abolished. rCsCCL21 enhanced the resistance of tongue sole against bacterial infection, but the mutant protein with NCCL mutation lacked this antibacterial effect. Taken together, these results suggest that CsCCL21 is a functional CC chemokine with the ability to recruit leukocytes and is involved in antibacterial immunity in a manner that requires the conserved NCCL motif.
趋化因子是一大类趋化性细胞因子。根据前两个半胱氨酸残基的排列方式，趋化因子可分为四类，其中一类是CC趋化因子组。在本研究中，我们鉴定了半滑舌鳎（Cynoglossus semilaevis）的一种CC趋化因子CsCCL21，并分析了其活性。CsCCL21在NCCL基序中含有两个保守的N端半胱氨酸残基，在系统发育上与CC趋化因子的CCL19/21/25亚组相关。CsCCL21在九个组织中组成性表达，并在细菌和病毒感染后显著上调。重组CsCCL21（rCsCCL21）诱导外周血白细胞迁移。当NCCL基序中的两个保守半胱氨酸残基发生突变时，rCsCCL21的趋化活性丧失。rCsCCL21增强了舌鳎对细菌感染的抵抗力，但具有NCCL突变的突变蛋白缺乏这种抗菌作用。综上所述，这些结果表明CsCCL21是一种具有募集白细胞能力的功能性CC趋化因子，并以一种需要保守NCCL基序的方式参与抗菌免疫。
