Rasheed Lubna, Yousuf Muhammad, Youn Il Seung, Yoon Taeseung, Kim Kwang-Youn, Seo Young-Kyo, Shi Genggongwo, Saleh Muhammad, Hur Jin-Hoe, Kim Kwang S
Center for Superfunctional Materials, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798 (Republic of Korea).
Department of Chemistry, Pohang University of Science and Technology, Pohang, 790-784 (Republic of Korea).
Chemistry. 2015 Nov 9;21(46):16349-53. doi: 10.1002/chem.201501892. Epub 2015 Sep 29.
Pyrene-based turn-on ratiometric fluorescent probe 1 demonstrates high sensitivity and exceptional selectivity toward Cr(3+) in the presence of other metals, including Fe(3+) in aqueous media. Interaction of Cr(3+) with probe 1 brings pyrene moieties close enough to have better aligned π-π stacking, thus enhancing the excimer peak many fold. On the other hand, the interaction of Fe(3+) with probe 1 brings forth a negligible difference in stacking, resulting in an insignificant change in fluorescence intensity. Exceptional selectivity of probe 1 with Cr(3+) over Fe(3+) and other metals has been confirmed by theoretical studies in addition to experimental results. Imaging of HeLa cells observed by confocal fluorescence microscopy reveals that probe 1 can be used to monitor Cr(3+) in live cells to map its subcellular distribution.
基于芘的开启型比率荧光探针1在水性介质中，在包括Fe(3+)在内的其他金属存在的情况下，对Cr(3+)表现出高灵敏度和出色的选择性。Cr(3+)与探针1的相互作用使芘基团足够靠近，以实现更好的π-π堆积排列，从而使准分子峰增强许多倍。另一方面，Fe(3+)与探针1的相互作用导致堆积差异可忽略不计，荧光强度变化不明显。除了实验结果外，理论研究也证实了探针1对Cr(3+)相对于Fe(3+)和其他金属具有出色的选择性。通过共聚焦荧光显微镜观察HeLa细胞成像表明，探针1可用于监测活细胞中的Cr(3+)，以绘制其亚细胞分布。
