Gu Xiaochu, Ni Peiyan, Xiang Bo, Zhao Liansheng, Wei Jinxue, Wang Yingche, Ma Xiaohong, Li Tao
Psychiatric Laboratory, Department of Psychiatry, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. Email:
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2015 Oct;32(5):625-8. doi: 10.3760/cma.j.issn.1003-9406.2015.05.004.
OBJECTIVE To explore the biological processes and pathways associated with memory function which may be regulated by gene promoter methylation. METHODS The genome-wide promoter methylation statuses in 9 healthy individuals were analyzed with a Multiplex HG18 CpG Promoter chip. Genes with promoter methylation statuses strongly correlated with both immediate and delayed visual memory function were preceded for pathway and physical interactions analysis. RESULTS Sixty nine genes have been correlated with both immediate and delayed visual memory functions. Twenty two pathways, with a Q-value of < 0.05, were identified by the pathway and physical interactions analysis, which included energy metabolism, axon guidance, tyrosine kinase activity, anterograde synaptic vesicle transport, and leukocyte migration and differentiation. CONCLUSION Pathways related with memory function may be regulated by DNA methylation.
目的 探讨可能受基因启动子甲基化调控的与记忆功能相关的生物学过程和信号通路。方法 采用多重HG18 CpG启动子芯片分析9名健康个体的全基因组启动子甲基化状态。对启动子甲基化状态与即时和延迟视觉记忆功能均密切相关的基因进行信号通路和物理相互作用分析。结果 69个基因与即时和延迟视觉记忆功能均相关。通过信号通路和物理相互作用分析，确定了22条Q值<0.05的信号通路，包括能量代谢、轴突导向、酪氨酸激酶活性、顺行性突触小泡运输以及白细胞迁移和分化。结论 与记忆功能相关的信号通路可能受DNA甲基化调控。
