Glanzman D L, Kandel E R, Schacher S
Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Neuron. 1989 Oct;3(4):441-50. doi: 10.1016/0896-6273(89)90203-1.
To determine the influence that an appropriate target cell has on the axonal structure of a presynaptic neuron in vivo, we examined the morphologies of individual Aplysia sensory neurons in dissociated cell culture in the presence or absence of identified target motor neurons. We find that an appropriate target, the motor cell L7, regulates the morphological differentiation of the presynaptic sensory neurons in two ways: the target induces the axons of the sensory neurons to develop a more elaborate structure and to form active zones, and the target guides the outgrowth of the sensory neurons. The influence of the appropriate target, L7, on the morphological differentiation of sensory neurons appears to be related to the formation of chemical synaptic connections between the sensory neurons and L7, since sensory neurons co-cultured with an inappropriate target motor neuron do not exhibit a comparable elaboration of their axonal processes.
为了确定合适的靶细胞在体内对突触前神经元轴突结构的影响，我们在解离细胞培养中，在存在或不存在已鉴定的靶运动神经元的情况下，检查了单个海兔感觉神经元的形态。我们发现，合适的靶细胞，即运动细胞L7，通过两种方式调节突触前感觉神经元的形态分化：靶细胞诱导感觉神经元的轴突发育出更精细的结构并形成活性区，并且靶细胞引导感觉神经元的生长。合适的靶细胞L7对感觉神经元形态分化的影响似乎与感觉神经元和L7之间化学突触连接的形成有关，因为与不合适的靶运动神经元共培养的感觉神经元其轴突过程没有表现出类似的精细变化。
