基于转录组序列开发的桑科波罗蜜属叶绿体微卫星标记。
Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences.
作者信息
Gardner Elliot M, Laricchia Kristen M, Murphy Matthew, Ragone Diane, Scheffler Brian E, Simpson Sheron, Williams Evelyn W, Zerega Nyree J C
机构信息
Department of Plant Science, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA ; Plant Biology and Conservation, Northwestern University, 2205 Tech Drive, Hogan 2-144, Evanston, Illinois 60208 USA.
Department of Biology, Illinois College, 1101 West College Avenue, Jacksonville, Illinois 62650 USA.
出版信息
Appl Plant Sci. 2015 Sep 10;3(9). doi: 10.3732/apps.1500049. eCollection 2015 Sep.
PREMISE OF THE STUDY
Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners.
METHODS AND RESULTS
Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome assemblies. The markers were evaluated using capillary electrophoresis in A. odoratissimus (105 accessions) and A. altilis (73). They were also evaluated in silico in A. altilis (10), A. camansi (6), and A. altilis × A. mariannensis (7) transcriptomes. All loci were polymorphic in at least one species, with all 15 polymorphic in A. camansi. Per species, average alleles per locus ranged between 2.2 and 2.5. Three loci had evidence of fragment-length homoplasy.
CONCLUSIONS
These markers will complement existing nuclear markers by enabling confident identification of maternal and clone lines, which are often important in vegetatively propagated crops such as breadfruit.
研究前提
从面包树（Artocarpus altilis）和面包坚果（A. camansi）的转录组中鉴定叶绿体微卫星位点。在香波罗蜜（A. odoratissimus）和面包树中对其进行测试，并对两个同属物种进行了电子评估。
方法与结果
在四个面包树属转录组组装的叶绿体序列中鉴定出15个简单序列重复（SSR）。使用毛细管电泳在香波罗蜜（105份材料）和面包树（73份材料）中对这些标记进行评估。还在面包树（10个）、面包坚果（6个）和面包树×大溪地面包树（A. altilis × A. mariannensis，7个）的转录组中进行了电子评估。所有位点在至少一个物种中具有多态性，在面包坚果中所有15个位点均为多态性。每个物种每个位点的平均等位基因数在2.2至2.5之间。三个位点存在片段长度同塑性的证据。
结论
这些标记将通过能够可靠地鉴定母系和克隆系来补充现有的核标记，这在面包果等无性繁殖作物中通常很重要。
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