Kalsoom Umme, Guijt Rosanne M, Boyce Mary C, Townsend Ashley T, Haselberg Rob, Breadmore Michael C
Australian Centre for Research on Separation Science (ACROSS), School of Physical Sciences Chemistry, University of Tasmania, Private Bag 75, Hobart, Tasmania, 7001, Australia; Centre for Ecosystem Management, School of Natural Sciences, Edith Cowan University, Perth, Western Australia, 6027, Australia.
School of Medicine and Australian Centre for Research on Separation Science (ACROSS), Private Bag 26, Hobart, Tasmania, 7001, Australia.
J Chromatogr A. 2016 Jan 8;1428:346-51. doi: 10.1016/j.chroma.2015.08.012. Epub 2015 Aug 28.
A novel approach for the direct injection from plant tissues without any sample pre-treatment has been developed by simply placing a small piece of the tissue into a capillary electrophoresis vial followed by application of a voltage for electrokinetic injection. Separations of sodium, potassium, calcium and magnesium were achieved using a BGE comprising 10mM imidazole and 2.5mM 18-crown-6-ether at pH 4.5. The addition of 2% (m/v) hydroxypropylmethyl cellulose to the separation buffer allowed for precise and accurate electrokinetic injection of ions from the plant material by halting the movement of tissue fluid into the capillary. This method provides both qualitative and quantitative data of inorganic cations, with quantitation in zucchini, mushroom and apple samples in agreement with Sector Field Inductively Coupled Plasma Mass Spectrometric analysis (r(2)=0.98, n=9). This method provides a new way for rapid, quantitative analysis by eliminating sample preparation procedures, and has great potential for a range of applications in plant science and food chemistry.
一种无需任何样品预处理即可直接从植物组织进行注射的新方法已经开发出来，该方法只需将一小片组织放入毛细管电泳瓶中，然后施加电压进行电动进样。使用pH值为4.5、由10mM咪唑和2.5mM 18-冠-6-醚组成的背景电解质实现了钠、钾、钙和镁的分离。向分离缓冲液中添加2%（m/v）羟丙基甲基纤维素，通过阻止组织液进入毛细管，实现了从植物材料中精确、准确地电动进样离子。该方法提供了无机阳离子的定性和定量数据，西葫芦、蘑菇和苹果样品中的定量结果与扇形场电感耦合等离子体质谱分析一致（r(2)=0.98，n=9）。该方法通过省去样品制备程序，为快速定量分析提供了一种新途径，在植物科学和食品化学的一系列应用中具有巨大潜力。
