Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P. R. China.
Food Funct. 2015 Dec;6(12):3799-806. doi: 10.1039/c5fo00815h.
The aim of this study is to isolate and identify a novel corn peptide (CP) from zein with antioxidant and antihypertensive activities based on bioactive-guided isolation procedures. Zein was hydrolyzed by using double enzymes immobilized with calcium alginate-chitosan beads and then fractionated and purified. The antioxidant and antihypertensive activities of the CP fractions were screened by in vitro and in vivo assays. The in vivo animal studies using spontaneously hypertensive rats (SHRs) confirmed the antihypertensive effects of the CP, and its angiotensin I-converting enzyme (ACE) inhibitory activity was retained after thermal treatment and simulated gastrointestinal digestion. The primary structure of CP-2-1 was identified by RP-HPLC-MS/MS and the amino acid sequence was determined as M-I/L-P-P with the molecular weight of 452.3 Da. CP-2-1 showed effective antioxidant and ACE inhibitory activities (IC50 values of 220 μg mL(-1) and 70.32 μg mL(-1), respectively) and it might be a potential candidate for antioxidant functional food or pharmaceuticals for hypertension.
本研究旨在通过生物活性导向分离程序，从玉米醇溶蛋白中分离并鉴定具有抗氧化和降血压活性的新型玉米肽（CP）。使用固定化在海藻酸钙-壳聚糖珠上的双酶水解玉米醇溶蛋白，然后进行分级和纯化。通过体外和体内试验筛选 CP 馏分的抗氧化和降血压活性。使用自发性高血压大鼠（SHRs）的体内动物研究证实了 CP 的降血压作用，并且其血管紧张素转化酶（ACE）抑制活性在热处理和模拟胃肠道消化后仍然保留。通过反相高效液相色谱-质谱/质谱（RP-HPLC-MS/MS）鉴定 CP-2-1 的一级结构，其氨基酸序列为 M-I/L-P-P，分子量为 452.3 Da。CP-2-1 表现出有效的抗氧化和 ACE 抑制活性（IC50 值分别为 220 μg mL（-1）和 70.32 μg mL（-1）），可能是抗氧化功能性食品或高血压药物的潜在候选物。
