Turk J Med Sci. 2015;45(4):800-3. doi: 10.3906/sag-1408-21.
BACKGROUND/AIM: The aim of this study was to compare the distribution of interstitial Cajal cells, eosinophils, and mast cells in normal and inflamed appendices, and to evaluate the correlation of presence of these cells with severity of inflammation in appendicitis.
The appendicitis group (n = 30) was divided further into three groups according to the macroscopic description and the histological findings. Ten normal appendices served as controls. Tissue samples were processed for routine histological examination. Additionally, all sections were immunohistochemically stained with CD117 and mast cell tryptase antibodies.
When specimens were compared in terms of Cajal cells, the observed mean number for the appendicitis group was 4.9 and for the control group it was 8.3. In contrast, eosinophils and mast cells were significantly increased in the appendicitis group when compared with the control group.
We detected that eosinophils and mast cells are increased in appendicitis, and correlate with the degree of inflammation of the appendix. The density of interstitial Cajal cells was significantly lower in patients with severe appendix inflammation compared to controls. The histopathological differences observed in this study may help elucidate the pathophysiology of appendicitis.
背景/目的：本研究旨在比较正常和炎症阑尾中间质 Cajal 细胞、嗜酸性粒细胞和肥大细胞的分布，并评估这些细胞的存在与阑尾炎炎症严重程度的相关性。
将阑尾炎组（n=30）进一步根据宏观描述和组织学发现分为三组。十个正常阑尾作为对照组。对组织样本进行常规组织学检查。此外，所有切片均用 CD117 和肥大细胞胰蛋白酶抗体进行免疫组织化学染色。
在 Cajal 细胞方面，观察到阑尾炎组的平均数量为 4.9，对照组为 8.3。相比之下，与对照组相比，在阑尾炎组中，嗜酸性粒细胞和肥大细胞显著增加。
我们发现嗜酸性粒细胞和肥大细胞在阑尾炎中增加，并与阑尾炎症的程度相关。与对照组相比，严重阑尾炎症患者的间质 Cajal 细胞密度显著降低。本研究中观察到的组织病理学差异可能有助于阐明阑尾炎的病理生理学。
