Kanji Alpa, Jones Michael A, Maskell Duncan J, Grant Andrew J
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, United Kingdom.
Institute for Animal Health, Compton, Newbury, Berkshire, United Kingdom.
Microb Pathog. 2015 Dec;89:93-9. doi: 10.1016/j.micpath.2015.09.010. Epub 2015 Sep 28.
Campylobacter jejuni is the leading cause of foodborne bacterial gastroenteritis worldwide. Although the mechanisms by which C. jejuni causes disease are not completely understood, the presence of functional flagella appears to be required for colonisation of the gastrointestinal tract of humans and animals. Therefore much attention has been given to understanding the synthesis and role of flagella in C. jejuni. In this study we report insights into the function of PflB that is essential for Campylobacter motility. We have explored the function of this gene by constructing deletion mutants in C. jejuni strains NCTC11168 and M1, in the genes cj0390 and CJM1_0368, respectively. The mutants were non-motile yet assembled flagella that appeared structurally identical to the wild type. Furthermore the protein is required for C. jejuni colonisation of caeca in a two-week old chicken colonisation model.
空肠弯曲菌是全球食源性细菌性肠胃炎的主要病因。尽管空肠弯曲菌致病的机制尚未完全明确，但功能性鞭毛的存在似乎是其在人和动物胃肠道定殖所必需的。因此，人们对了解空肠弯曲菌中鞭毛的合成及作用给予了极大关注。在本研究中，我们报告了对PflB功能的见解，PflB对弯曲菌的运动至关重要。我们通过在空肠弯曲菌菌株NCTC11168和M1中分别构建cj0390和CJM1_0368基因的缺失突变体，来探究该基因的功能。这些突变体不具有运动能力，但能组装出结构上与野生型相同的鞭毛。此外，在两周龄鸡的定殖模型中，该蛋白是空肠弯曲菌在盲肠定殖所必需的。
