Baldigo Barry P, George Scott D, Phillips Patrick J, Hemming Jocelyn D C, Denslow Nancy D, Kroll Kevin J
New York Water Science Center, US Geological Survey, Troy, New York, USA.
Wisconsin State Laboratory of Hygiene, Madison, Wisconsin, USA.
Environ Toxicol Chem. 2015 Dec;34(12):2803-15. doi: 10.1002/etc.3120. Epub 2015 Oct 29.
Direct linkages between endocrine-disrupting compounds (EDCs) from municipal and industrial wastewaters and impacts on wild fish assemblages are rare. The levels of plasma vitellogenin (Vtg) and Vtg messenger ribonucleic acid (mRNA) in male fathead minnows (Pimephales promelas) exposed to wastewater effluents and dilutions of 17α-ethinylestradiol (EE2), estrogen activity, and fish assemblages in 10 receiving streams were assessed to improve understanding of important interrelations. Results from 4-d laboratory assays indicate that EE2, plasma Vtg concentration, and Vtg gene expression in fathead minnows, and 17β-estradiol equivalents (E2Eq values) were highly related to each other (R(2) = 0.98-1.00). Concentrations of E2Eq in most effluents did not exceed 2.0 ng/L, which was possibly a short-term exposure threshold for Vtg gene expression in male fathead minnows. Plasma Vtg in fathead minnows only increased significantly (up to 1136 μg/mL) in 2 wastewater effluents. Fish assemblages were generally unaffected at 8 of 10 study sites, yet the density and biomass of 79% to 89% of species populations were reduced (63-68% were reduced significantly) in the downstream reach of 1 receiving stream. These results, and moderate to high E2Eq concentrations (up to 16.1 ng/L) observed in effluents during a companion study, suggest that estrogenic wastewaters can potentially affect individual fish, their populations, and entire fish communities in comparable systems across New York, USA.
城市和工业废水中的内分泌干扰化合物（EDC）与对野生鱼类群落的影响之间的直接联系很少见。评估了暴露于废水排放物和17α-乙炔雌二醇（EE2）稀释液中的雄性黑头呆鱼（Pimephales promelas）的血浆卵黄蛋白原（Vtg）和Vtg信使核糖核酸（mRNA）水平、雌激素活性以及10条接纳溪流中的鱼类群落，以增进对重要相互关系的理解。为期4天的实验室分析结果表明，EE2、黑头呆鱼的血浆Vtg浓度和Vtg基因表达以及17β-雌二醇当量（E2Eq值）彼此高度相关（R(2) = 0.98 - 1.00）。大多数排放物中的E2Eq浓度不超过2.0 ng/L，这可能是雄性黑头呆鱼Vtg基因表达的短期暴露阈值。只有2种废水排放物中的黑头呆鱼血浆Vtg显著增加（高达1136 μg/mL）。在10个研究地点中的8个，鱼类群落通常未受影响，但在1条接纳溪流的下游河段，79%至89%的物种种群密度和生物量降低（63% - 68%显著降低）。这些结果，以及在一项配套研究中在排放物中观察到的中度至高度E2Eq浓度（高达16.1 ng/L），表明雌激素废水可能会影响美国纽约类似系统中的个体鱼类、其种群以及整个鱼类群落。
