Departament de Química Analítica i Institut de Biomedicina (IBUB), Universitat de Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain.
Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium.
Anal Chim Acta. 2015 Sep 24;894:20-34. doi: 10.1016/j.aca.2015.08.030. Epub 2015 Aug 28.
Due to the developing insights in the theory of chromatography, column manufacturers of any kind (industrial, academic) nowadays have a broad array of experimental column testing tools at their disposal. The present tutorial aims at helping the novice in the field getting an overview of these tools and provides a fixed procedure to carry out the subsequent steps in the column quality analysis (guided via an Excel template file). After some brief introduction about the main equations, the reader is taken step by step through the theories underlying the measurement methods for the different column and performance parameters. In the final section, the reader is taken through the different items in the Excel template.
由于色谱理论的不断发展，如今各种类型的（工业、学术）柱制造商都可以使用广泛的实验柱测试工具。本教程旨在帮助该领域的新手概述这些工具，并提供执行柱质量分析后续步骤的固定程序（通过 Excel 模板文件进行指导）。在简要介绍主要方程之后，读者将逐步了解不同柱和性能参数的测量方法的理论基础。在最后一节中，读者将了解 Excel 模板中的不同项目。
