Prabhala Bala K, Mirza Osman, Højrup Peter, Hansen Paul R
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Methods Mol Biol. 2015;1348:77-82. doi: 10.1007/978-1-4939-2999-3_9.
Mass spectrometry (MS) is well suited for analysis of the identity and purity of synthetic peptides. The sequence of a synthetic peptide is most often known, so the analysis is mainly used to confirm the identity and purity of the peptide. Here, simple procedures are described for MALDI-TOF-MS and LC-MS of synthetic peptides.
质谱分析法（MS）非常适合用于分析合成肽的身份和纯度。合成肽的序列通常是已知的，因此该分析主要用于确认肽的身份和纯度。本文描述了用于合成肽的基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF-MS）和液相色谱-质谱联用（LC-MS）的简单程序。
