锌指蛋白SPT4与SPT5L/KTF1相互作用并调节拟南芥中的转录沉默。
The zinc-finger protein SPT4 interacts with SPT5L/KTF1 and modulates transcriptional silencing in Arabidopsis.
作者信息
Köllen Karin, Dietz Lena, Bies-Etheve Natacha, Lagrange Thierry, Grasser Marion, Grasser Klaus D
机构信息
Cell Biology & Plant Biochemistry, Biochemie-Zentrum Regensburg, University of Regensburg, Universitätsstr. 31, D-93053 Regensburg, Germany.
Laboratoire Génome et Développement des Plantes, Université de Perpignan, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France.
出版信息
FEBS Lett. 2015 Oct 24;589(21):3254-7. doi: 10.1016/j.febslet.2015.09.017. Epub 2015 Sep 28.
The Arabidopsis multidomain protein SPT5L/KTF1 (which has similarity to the transcript elongation factor SPT5) associates with RNA polymerase V (RNAPV) and is an accessory factor in RNA-directed DNA methylation. The zinc-finger protein SPT4 was found to interact with SPT5L (and SPT5) both in vivo and in vitro. Here, we show that plants depleted of SPT4 relative to wild type display reduced DNA methylation and the locus specificity is shared with SPT5L, suggesting a cooperation of SPT4 and SPT5L. Unlike observed for SPT5, no reduced protein level of SPT5L is determined in SPT4-deficient plants. These experiments demonstrate that in addition to the RNA polymerase II-associated SPT4/SPT5 that is generally conserved in eukaryotes, flowering plants have SPT4/SPT5L that is involved in RNAPV-mediated transcriptional silencing.
拟南芥多结构域蛋白SPT5L/KTF1（与转录延伸因子SPT5具有相似性）与RNA聚合酶V（RNAPV）相关联，并且是RNA指导的DNA甲基化中的一个辅助因子。锌指蛋白SPT4在体内和体外均被发现与SPT5L（以及SPT5）相互作用。在这里，我们表明相对于野生型而言，缺失SPT4的植物显示出DNA甲基化降低，并且位点特异性与SPT5L相同，这表明SPT4和SPT5L存在协同作用。与SPT5的情况不同，在缺乏SPT4的植物中未检测到SPT5L的蛋白质水平降低。这些实验表明，除了在真核生物中普遍保守的与RNA聚合酶II相关的SPT4/SPT5之外，开花植物还具有参与RNAPV介导的转录沉默的SPT4/SPT5L。
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