Alvarez Angel H, Gutiérrez-Ortega Abel, Hernández-Gutiérrez Rodolfo
Medical and Pharmaceutical Biotechnology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, Colinas de la Normal, 44270 Guadalajara, Jalisco, México.
Can J Vet Res. 2015 Oct;79(4):339-42.
Mannheimia haemolytica and Histophilus somni are frequently isolated from diseased cattle with bovine respiratory disease (BRD). They compromise animal lung function and the immune responses generated are not sufficient to limit infection. Identification of specific immunogenic antigens for vaccine development represents a great challenge. Immunogenic proteins were identified by immunoproteomic approach with sera from cattle immunized with a commercial cellular vaccine of M. haemolytica and H. somni. Proteins of M. haemolytica were identified as solute ABC transporter, iron-binding protein, and hypothetical protein of capsular biosynthesis. Histophilus somni proteins correspond to porin, amino acid ABC transporter, hypothetical outer membrane protein, cysteine synthase, and outer membrane protein P6. Although these antigens share strong similarities with other proteins from animal pathogens, the ABC system proteins have been associated with virulence and these proteins could be considered as potential vaccine candidates for BRD.
溶血曼氏杆菌和睡眠嗜组织菌经常从患有牛呼吸道疾病（BRD）的病牛中分离出来。它们损害动物肺功能，并且产生的免疫反应不足以限制感染。鉴定用于疫苗开发的特定免疫原性抗原是一项巨大挑战。通过免疫蛋白质组学方法，用溶血曼氏杆菌和睡眠嗜组织菌的商业细胞疫苗免疫牛的血清鉴定免疫原性蛋白质。溶血曼氏杆菌的蛋白质被鉴定为溶质ABC转运蛋白、铁结合蛋白和荚膜生物合成的假定蛋白。睡眠嗜组织菌的蛋白质对应于孔蛋白、氨基酸ABC转运蛋白、假定外膜蛋白、半胱氨酸合酶和外膜蛋白P6。尽管这些抗原与动物病原体的其他蛋白质有很强的相似性，但ABC系统蛋白已与毒力相关，这些蛋白质可被视为BRD潜在的疫苗候选物。
