Krager Kimberly J, Pineda E Nathalie, Kharade Sujay V, Kordsmeier Mary, Howard Luke, Breen Philip J, Compadre Cesar M, Hauer-Jensen Martin, Aykin-Burns Nukhet
Division of Radiation Health, . University of Arkansas for Medical Sciences, 4301 West Markham, No. 522-10, Little Rock, AR 72205, USA ; Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 4301 West Markham, No. 522-10, Little Rock, AR 72205, USA.
Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 4301 West Markham, No. 522-10, Little Rock, AR 72205, USA.
Evid Based Complement Alternat Med. 2015;2015:148791. doi: 10.1155/2015/148791. Epub 2015 Aug 26.
The vitamin E analogs δ-tocotrienol (DT3) and γ-tocotrienol (GT3) have significant protective and mitigative capacity against the detrimental effects of ionizing radiation (IR). However, the expense of purification limits their potential use. This study examined the tocotrienol-rich fraction of rice bran (TRFRB) isolated from rice bran deodorizer distillate, a rice oil refinement waste product, to determine its protective effects against IR induced oxidative damage and H2O2. Several cell lines were treated with tocotrienols or TRFRB prior to or following exposure to H2O2 or IR. To determine the radioprotective capacity cells were analyzed for morphology, mitochondrial bioenergetics, clonogenic survival, glutathione oxidation, cell cycle, and migration rate. TRFRB displayed similar antioxidant activity compared to pure tocotrienols. Cells pretreated with TRFRB or DT3 exhibited preserved cell morphology and mitochondrial respiration when exposed to H2O2. Oxidized glutathione was decreased in TRFRB treated cells exposed to IR. TRFRB reversed mitochondrial uncoupling and protected cells migration rates following IR exposure. The protective antioxidant capacity of TRFRB treated cells against oxidative injury was similar to that of purified DT3. TRFRB effectively protects normal cells against IR induced injury suggesting that rice bran distillate may be an inexpensive and abundant alternate source.
维生素E类似物δ-生育三烯酚（DT3）和γ-生育三烯酚（GT3）对电离辐射（IR）的有害影响具有显著的保护和减轻作用。然而，纯化成本限制了它们的潜在用途。本研究检测了从米糠脱臭馏出物（一种米油精炼废品）中分离得到的富含生育三烯酚的米糠馏分（TRFRB），以确定其对IR诱导的氧化损伤和H2O2的保护作用。在暴露于H2O2或IR之前或之后，用生育三烯酚或TRFRB处理几种细胞系。为了确定辐射防护能力，分析细胞的形态、线粒体生物能量学、克隆形成存活率、谷胱甘肽氧化、细胞周期和迁移率。与纯生育三烯酚相比，TRFRB表现出相似的抗氧化活性。用TRFRB或DT3预处理的细胞在暴露于H2O2时表现出保留的细胞形态和线粒体呼吸。在暴露于IR的TRFRB处理的细胞中，氧化型谷胱甘肽减少。TRFRB逆转了线粒体解偶联，并在IR暴露后保护细胞迁移率。TRFRB处理的细胞对氧化损伤的抗氧化保护能力与纯化的DT3相似。TRFRB有效地保护正常细胞免受IR诱导的损伤，表明米糠馏出物可能是一种廉价且丰富的替代来源。
