Nikandrov V V, Brin G P, Krasnovskiĭ A A
Biokhimiia. 1978;43(4):636-45.
Illumination of NADH and NADPH by UV-light in the absence of oxygen resulted in the reduction of ferredoxin or methyl-viologen to cation-radical and under prolonged illumination to dihydrodipyridyl. The reaction may by accompanied by triplet and singlet exitation of NADH. It was shown that hematoporphyrin in aqueous solution photosensitized the reaction of NADH oxidation by ferredoxin and methylviologen to the visible region of the spectrum. Under light excitation the redox potentials of NADH and NADPH were increased up to the level exceeding the potential of hydrogen electrode. Illumination of NADH and NADPH by UV-light in the presence of bacterial hydrogenase resulted in hydrogen evolution. The reaction of hydrogen evolution could be sensitised towards the visible region of the spectrum by chlorophyll or chloroplasts.
在无氧条件下，用紫外光照射NADH和NADPH会导致铁氧还蛋白或甲基紫精还原为阳离子自由基，长时间照射则会还原为二氢二吡啶。该反应可能伴随着NADH的三线态和单线态激发。结果表明，水溶液中的血卟啉可将铁氧还蛋白和甲基紫精氧化NADH的反应光敏化至光谱的可见光区域。在光激发下，NADH和NADPH的氧化还原电位升高至超过氢电极电位的水平。在细菌氢化酶存在的情况下，用紫外光照射NADH和NADPH会产生氢气。叶绿素或叶绿体可使产氢反应对光谱的可见光区域敏感。
