子宫内膜及子宫内膜异位症病灶中干细胞相关基因的表达：一项初步研究。
Expression of stem cell-related genes in the endometrium and endometriotic lesions: a pilot study.
作者信息
Fettback Paula B T, Pereira Ricardo M A, Rocha André M, Soares José Maria, Smith Gary D, Baracat Edmund C, Serafini Paulo C
机构信息
a Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina da Universidade de São Paulo, Hospital das Clínicas , São Paulo , Brazil .
b Huntington Centro de Medicina Reprodutiva , São Paulo , Brazil , and.
出版信息
Gynecol Endocrinol. 2016;32(1):82-6. doi: 10.3109/09513590.2015.1092135. Epub 2015 Oct 1.
OBJECTIVE
To compare the expression of stem cell-related genes in the endometrium (END), superficial endometriosis (SE), and deep infiltrating endometriosis (DIE).
STUDY DESIGN
We performed a prospective pilot study of six women suffering from SE and DIE who gave consent for laparoscopy surgery, endometrial biopsies, and participation in this study. Quantitative RT-PCR analysis of 84 stem cell-related genes was performed in 18 biopsy samples.
RESULTS
A total of 40 of 84 genes were expressed in SE and DIE, but were different from END as follows. Seven genes were over-expressed in SE and 33 genes were under-expressed in DIE compared with END. Two genes were only over-expressed in SE and three genes were only over-expressed in DIE. Six under-expressed genes were exclusively located in SE and one was only located in DIE. The remaining 31 genes were not different among the groups. There was no significant difference in gene expression between SE and DIE samples.
CONCLUSION
Tissue of DIE and SE appears to have similar stem cell-related genes. Nevertheless, there are differences in gene expression between SE and DIE.
目的
比较子宫内膜（END）、浅表性子宫内膜异位症（SE）和深部浸润性子宫内膜异位症（DIE）中干细胞相关基因的表达情况。
研究设计
我们对6名患有SE和DIE且同意接受腹腔镜手术、子宫内膜活检并参与本研究的女性进行了一项前瞻性试点研究。对18份活检样本进行了84种干细胞相关基因的定量逆转录聚合酶链反应（RT-PCR）分析。
结果
84种基因中共有40种在SE和DIE中表达，但与END不同，情况如下。与END相比，7种基因在SE中过度表达，33种基因在DIE中低表达。2种基因仅在SE中过度表达，3种基因仅在DIE中过度表达。6种低表达基因仅存在于SE中，1种仅存在于DIE中。其余31种基因在各组之间无差异。SE和DIE样本之间的基因表达无显著差异。
结论
DIE和SE组织似乎具有相似的干细胞相关基因。然而，SE和DIE之间在基因表达上存在差异。
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