Clin Chem Lab Med. 2016 Mar;54(3):419-25. doi: 10.1515/cclm-2015-0650.
Endogenous interferences are an important source of biased laboratory results. Hemolysis, lipemia and icteria are the main source of endogenous interference in laboratory medicine. Accreditation according to ISO 15189 improves the overall quality of the laboratory procedures. The aim of our study was i) to assess the level of knowledge of Croatian medical biochemists about the proper detection and management of hemolysis, lipemia and icteria; and ii) to identify possible differences in the level of knowledge respective to the laboratory accreditation status.
An on-line self-report survey was carried out by the Working Group for Preanalytical Phase of the Croatian Society of Medical Biochemistry and Laboratory Medicine during April to May 2015. Survey included 14 statements (Q1-Q14) about procedures for samples with interferences and participants were asked to assess the degree of agreement with the statement using a 4-point Likert scale.
The lowest level of knowledge was observed for statements Q10 (dealing with icteric sample; 40.9% participants agreed with the correct procedure), Q12 (allowable error for interference; 47.2%) and Q11 (dealing with lipemic sample; 60.1%). Almost all participants (97.4%) agreed that laboratories in Croatia should have a harmonized protocol for management of samples with interferences. Participants from accredited laboratories showed higher knowledge of hemolysis detection (p=0.031), rejection of hemolyzed sample (p<0.001), management of icteric samples (p=0.038) and allowable error for interferences (p=0.040).
Croatian laboratories have a good knowledge of the proper detection and management of hemolyzed, icteric and lipemic samples. Accreditation is associated with higher knowledge about management of samples with interferences.
内源性干扰是实验室结果产生偏差的一个重要来源。溶血、脂血和黄疸是实验室医学中内源性干扰的主要来源。按照 ISO15189 进行认证可提高实验室程序的整体质量。我们研究的目的是：i）评估克罗地亚医学生物化学家和实验室管理人员对溶血、脂血和黄疸的正确检测和管理的认知水平；ii）确定其知识水平与实验室认证状态的差异。
2015 年 4 月至 5 月期间，克罗地亚医学生物化学和实验室医学协会的前分析阶段工作组通过在线自我报告调查进行了这项研究。该调查包括 14 个关于有干扰样本的程序的陈述（Q1-Q14），参与者被要求使用 4 分李克特量表评估对陈述的认同程度。
对处理黄疸样本的陈述 Q10（40.9%的参与者同意正确程序）、允许干扰的误差的陈述 Q12（47.2%）和处理脂血样本的陈述 Q11（60.1%）的知识水平最低。几乎所有参与者（97.4%）都同意克罗地亚的实验室应该有一个关于管理有干扰样本的协调一致的方案。来自认证实验室的参与者对溶血检测（p=0.031）、拒收溶血样本（p<0.001）、管理黄疸样本（p=0.038）和允许干扰的误差（p=0.040）的知识水平较高。
克罗地亚的实验室对溶血、黄疸和脂血样本的正确检测和管理有较好的认识。认证与对管理有干扰样本的较高认识相关。
