Xu Guangyu, Wang Jianmeng, Guo Yuqi, Bai Yu, Yuan Guangxin, An Liping, Han Xiao, Du Peige, Zhang Nan, Zheng Yan
Clin Lab. 2015;61(8):1017-25. doi: 10.7754/clin.lab.2015.141211.
Tuberculosis (TB) is a serious communicable disease throughout the world. Re-emergence of the TB epidemic is aggravated by the circulation of multidrug-resistant Mycobacterium tuberculosis strains, and more than half of new cases have occurred in Asia. Therefore, it is important to understand the gene mutations underlying the development of rifampicin resistance in Asia.
In this study, we classified the rifampicin-resistant Mycobacterium tuberculosis (MTB) rpoB data downloaded from Genbank, based on 12 mutation points. The relationship between the mutation sites and regional information was analyzed, after which the mutation dates and mutation trends of the rpoB gene were predicted by the Markov Chain Monte Carlo (MCMC) method.
We discovered that the mutation sites of the rpoB gene were disparate in different regions of Asia. The results of this study clearly showed that drug-resistant gene mutations in Asia started to increase in 2000 and peaked in 2006, indicating the relationship between drug resistance and outbreak trends of TB.
From our analysis, it was not difficult to see the relationship between the mutation rates of the rpoB gene and the outbreak of TB. Hence, to some degree, outbreak trends of TB can be predicted through genotyping based on the rpoB gene.
结核病是一种在全球范围内严重的传染病。耐多药结核分枝杆菌菌株的传播加剧了结核病疫情的再次出现，并且超过一半的新病例发生在亚洲。因此，了解亚洲利福平耐药性产生背后的基因突变很重要。
在本研究中，我们基于12个突变点对从Genbank下载的耐利福平结核分枝杆菌（MTB）rpoB数据进行分类。分析了突变位点与区域信息之间的关系，之后通过马尔可夫链蒙特卡罗（MCMC）方法预测了rpoB基因的突变日期和突变趋势。
我们发现rpoB基因的突变位点在亚洲不同地区存在差异。本研究结果清楚地表明，亚洲的耐药基因突变在2000年开始增加，并在2006年达到峰值，这表明了耐药性与结核病爆发趋势之间的关系。
从我们的分析中不难看出rpoB基因的突变率与结核病爆发之间的关系。因此，在某种程度上，可以通过基于rpoB基因的基因分型来预测结核病的爆发趋势。
