Wilson R E, Dooley T P, Hart I R
Biology of Metastasis, Imperial Cancer Research Fund Laboratories, London, United Kingdom.
Cancer Res. 1989 Feb 1;49(3):711-6.
A nontumorigenic line of murine melanocytes, Mel-ab, has been transfected with the v-Ha-ras gene under transcriptional control of the Moloney murine leukemia virus long terminal repeat. Transfectants produced rapidly growing undifferentiated melanomas in recipient mice. The inhibition of melanin production in transformed cells, observable both in vitro and in vivo, suggests that ras may affect melanocyte cytodifferentiation. Mel-ab cells require the continual presence of 12-O-tetradecanoylphorbol-13-acetate, or other activators of protein kinase C, for in vitro growth. Transfectants expressing v-Ha-ras no longer manifested this requirement and were actually growth inhibited by the addition of protein kinase C activators. These results are consistent with the notion that ras acts via the protein kinase C pathway in conferring autonomous growth on Mel-ab cells.
一种非致瘤性的小鼠黑素细胞系Mel-ab，已在莫洛尼鼠白血病病毒长末端重复序列的转录控制下用v-Ha-ras基因进行了转染。转染子在受体小鼠中产生了快速生长的未分化黑色素瘤。在体外和体内均可观察到，转化细胞中黑色素生成受到抑制，这表明ras可能影响黑素细胞的细胞分化。Mel-ab细胞在体外生长需要持续存在12-O-十四酰佛波醇-13-乙酸酯或蛋白激酶C的其他激活剂。表达v-Ha-ras的转染子不再表现出这种需求，实际上添加蛋白激酶C激活剂会抑制其生长。这些结果与ras通过蛋白激酶C途径赋予Mel-ab细胞自主生长能力的观点一致。
