Hazim Roni, Jiang Mei, Esteve-Rudd Julian, Diemer Tanja, Lopes Vanda S, Williams David S
UCLA School of Medicine, Jules Stein Eye Institute, 100 Stein Plaza, 90095, Los Angeles, CA, USA.
Adv Exp Med Biol. 2016;854:751-5. doi: 10.1007/978-3-319-17121-0_100.
The retinal pigment epithelium (RPE) is a post-mitotic epithelial monolayer situated between the light-sensitive photoreceptors and the choriocapillaris. Given its vital functions for healthy vision, the RPE is a primary target for insults that result in blinding diseases, including age-related macular degeneration (AMD). One such function is the phagocytosis and digestion of shed photoreceptor outer segments. In the present study, we examined the process of trafficking of outer segment disk membranes in live cultures of primary mouse RPE, using high speed spinning disk confocal microscopy. This approach has enabled us to track phagosomes, and determine parameters of their motility, which are important for their efficient degradation.
视网膜色素上皮（RPE）是位于光敏感光感受器和脉络膜毛细血管之间的有丝分裂后单层上皮。鉴于其对健康视力的重要功能，RPE是导致致盲疾病（包括年龄相关性黄斑变性（AMD））的损伤的主要靶点。其中一项功能是吞噬和消化脱落的光感受器外节。在本研究中，我们使用高速旋转盘共聚焦显微镜检查了原代小鼠RPE活培养物中外节盘膜的运输过程。这种方法使我们能够追踪吞噬体，并确定其运动参数，这些参数对其有效降解很重要。
