Shabani R, Ashtari K, Behnam B, Izadyar F, Asgari H, Asghari Jafarabadi M, Ashjari M, Asadi E, Koruji M
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Andrologia. 2016 Jun;48(5):584-94. doi: 10.1111/and.12490. Epub 2015 Oct 1.
Testicular cancer is the most common cancer affecting men in reproductive age, and cisplatin is one of the major helpful chemotherapeutic agents for treatment of this cancer. In addition, exposure of testes cancer cells to cisplatin could potentially eliminate tumour cells from germ cells in patients. The aim of this study was to evaluate the effect of cisplatin on viability of mouse acute lymphoblastic leukaemia cell line (EL-4) and neonatal mouse spermatogonial cells in vitro. In this study, the isolated spermatogonial stem cells (SSC) and EL-4 were divided into six groups including control (received medium), sham (received DMSO in medium) and experimental groups which received different doses of cisplatin (0.5, 5, 10 and 15 μg ml(-1) ). Cells viability was evaluated with MTT assay. The identity of the cultured cells was confirmed by the expression of specific markers. Our finding showed that viability of both SSC and EL-4 cells was reduced with the dose of 15 μg/ml when compared to the control group (P ≤ 0.05). Also, the differences between the IC50 in doses 10 and 15 μg/ml at different time were significant (P ≤ 0.05). The number of TUNEL-positive cells was increased, and the BAX and caspase-3 expressions were upregulated in EL4 cells for group that received an effective dose of cisplatin). In conclusion, despite the dramatic effects of cisplatin on both cells, spermatogonial stem cells could form colony in culture.
睾丸癌是影响育龄男性的最常见癌症，顺铂是治疗这种癌症的主要有效化疗药物之一。此外，将睾丸癌细胞暴露于顺铂可能会从患者的生殖细胞中消除肿瘤细胞。本研究的目的是评估顺铂对小鼠急性淋巴细胞白血病细胞系（EL-4）和新生小鼠精原细胞体外活力的影响。在本研究中，分离的精原干细胞（SSC）和EL-4被分为六组，包括对照组（接受培养基）、假手术组（接受培养基中的二甲基亚砜）和接受不同剂量顺铂（0.5、5、10和15μg/ml）的实验组。用MTT法评估细胞活力。通过特异性标志物的表达确认培养细胞的身份。我们的研究结果表明，与对照组相比，当顺铂剂量为15μg/ml时，SSC和EL-4细胞的活力均降低（P≤0.05）。此外，在不同时间，10μg/ml和15μg/ml剂量的半数抑制浓度（IC50）之间的差异具有统计学意义（P≤0.05）。在接受有效剂量顺铂的EL4细胞组中，TUNEL阳性细胞数量增加，BAX和半胱天冬酶-3表达上调。总之，尽管顺铂对两种细胞都有显著影响，但精原干细胞在培养中仍可形成集落。
